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Invitrogen
Bovine Serum Albumin Polyclonal Antibody
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Bovine Serum Albumin Antibody (A11133) in WB
Western blot analysis was performed by loading 500 ng (Lane 1), 250 ng (Lane 2), 125 ng (Lane 3) and 60 ng (Lane 4) of BSA. The blot was probed with Anti- Bovine Serum Albumin Rabbit Polyclonal Antibody (Product # A11133, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). ... View More
产品信息
A11133
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine
已发表种属
Baboon,
Bovine,
C. elegans,
Human,
Mouse,
Non-human primate,
Rabbit
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Bovine Serum Albumin
偶联物
形式
Liquid
浓度
2 mg/mL
纯化类型
IgG fraction
保存液
PBS, pH 7.2
内含物
5mM sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Anti-bovine serum albumin (anti-BSA) antibody is a highly purified rabbit IgG fraction that can be used for the detection of BSA in tracer studies or in protease assays. This antibody demonstrates high specificity for BSA and little or no crossreactivity to other serum proteins. Cat. No. A11133 can be used in enzyme and fluorescence immunoassays and blots, cell or tissue immunostaining, and as a precipitating antibody or as an immuno-adsorbant when attached to solid supports. For detection of this rabbit antibody, we offer a variety of second-step reagents labeled with biotin or with a wide range of fluorophores.
Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. We suggest using serial dilutions from about 1:100 to 1:1,000 to determine the optimal conditions for your experiments.
靶标信息
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Albumin; bovine albumin; BSA; MGC179277; serum albumin
基因别名: ALB
UniProt ID: (Bovine) A5PJX3
Entrez Gene ID: (Bovine) 280717
