Invitrogen

Brachyury Monoclonal Antibody (6C12)

查看其他33个Brachyury抗体

问题与答案

问题与答案

抗体检测数据 (4)

Brachyury Antibody in Western Blot (WB)

Brachyury Antibody in Western Blot (WB)

图: 1 / 4

Brachyury Antibody (MA5-31920) in WB

Western blot analysis of T in HEK293 (lane 1) and T (AA: 218-352)-hIgGFc transfected HEK293 cell lysate (lane 2). Samples were incubated with T monoclonal antibody (Product # MA5-31920) using a dilution of 1:500-1:2000.

Brachyury Antibody in Western Blot (WB)

Brachyury Antibody in Western Blot (WB)

Brachyury Antibody in Flow Cytometry (Flow)

Brachyury Antibody in ELISA (ELISA)

Product Image Gallery

Brachyury Antibody in Western Blot (WB) — Western blot analysis of T in HEK293 (lane 1) and T (AA: 218-352)-hIgGFc transfected HEK293 cell lysate (lane 2). Samples were incubated with T monoclonal antibody (Product # MA5-31920) using a dilution of 1:500-1:2000.

Brachyury Antibody in Western Blot (WB) — Western blot analysis of human T recombinant protein. Sample was incubated with T monoclonal antibody (Product # MA5-31920) using a dilution of 1:500-1:2000.

Brachyury Antibody in Flow Cytometry (Flow) — Flow cytometry of T in A549 cells (green). Sample was incubated with T monoclonal antibody (Product # MA5-31920) using a dilution of 1:200-1:400 followed by negative control (red).

Brachyury Antibody in ELISA (ELISA) — ELISA analysis of T in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with T monoclonal antibody (Product # MA5-31920) using a dilution of 1:10,000.

Immunogen image

Brachyury Monoclonal Antibody (6C12)

Immunogen Image Brachyury Monoclonal Antibody (6C12)

产品信息

MA5-31920

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

流式细胞分析 (Flow)

1:200-1:400

-

酶联免疫吸附实验 (ELISA)

1:10,000

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

6C12

抗原

Purified recombinant fragment of human T (AA: 218-352) expressed in E. coli.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

MA5-31920 has been tested in indirect ELISA.

靶标信息

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Brachyury protein; Protein T; RP11-459F1.1; T brachyury homolog; T, brachyury homolog; T-box transcription factor T

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基因别名: SAVA; T; TBXT; TFT

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UniProt ID: (Human) O15178

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Entrez Gene ID: (Human) 6862

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RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Rel homology transcription factor

negative regulation of transcription from RNA polymerase II promoter vasculogenesis somitogenesis neural plate morphogenesis neural tube closure heart morphogenesis positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation transcription, DNA-templated signal transduction penetration of zona pellucida mesoderm development mesoderm migration involved in gastrulation positive regulation of cell proliferation anterior/posterior axis specification, embryo notochord formation signal transduction involved in regulation of gene expression BMP signaling pathway post-anal tail morphogenesis embryonic skeletal system development canonical Wnt signaling pathway bone morphogenesis SMAD protein signal transduction determination of heart left/right asymmetry cellular response to retinoic acid primitive streak formation