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Invitrogen
BrdU Monoclonal Antibody (BU20A), PE, eBioscience™
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BrdU Antibody (12-5071-42) in Flow
Intracellular staining of 2-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0031-82 and Product # 16-0281-82)-stimulated C57Bl/6 splenocytes using the Foxp3/Transcription Staining Buffer Set (Product # 00-5523-00) with Anti-Mouse CD4 FITC (Product # 11-0042-82) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-BrdU PE (right). Cells in the lymphocyte gate were used for analysis.
产品信息
12-5071-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
已发表种属
Chemical
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
BU20A
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: This Bu20a monoclonal antibody reacts with 5-bromodeoxyuridine (BrdU). BrdU is a derivative of uridine that can be incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Anti-BrdU can then be used to identify cells that have undergone DNA synthesis during BrdU treatment.
For staining for flow cytometric analysis, we recommend the use of the BrdU Staining Buffer Set (Product # 00-5525) and protocol.
Applications Reported: This BU20A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This BU20A antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Staining Buffer Set (Product # 00-5523-00) or the BrdU Staining Buffer Set (Product # 00-5525-0) and protocol. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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