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Invitrogen
BrdU Monoclonal Antibody (BU20A)
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产品信息
MA1-81890
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
已发表种属
Chemical,
Mouse
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
BU20A
抗原
Bromodeoxyuridine conjugated to BSA.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd).
靶标信息
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
