Invitrogen

C1orf35 Polyclonal Antibody

查看其他3个MMTAG2抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (4)

C1orf35 Antibody in Immunocytochemistry (ICC/IF)

C1orf35 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

C1orf35 Antibody (PA5-99222) in ICC/IF

Immunocytochemistry analysis of C1orf35 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with C1orf35 polyclonal antibody (Product # PA5-99222) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

C1orf35 Antibody in Immunocytochemistry (ICC/IF)

C1orf35 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

C1orf35 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

C1orf35 Antibody in Western Blot (WB)

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C1orf35 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of C1orf35 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with C1orf35 polyclonal antibody (Product # PA5-99222) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

C1orf35 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of C1orf35 in human gastric cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with C1orf35 polyclonal antibody (Product # PA5-99222) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

C1orf35 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of C1orf35 in rat brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with C1orf35 polyclonal antibody (Product # PA5-99222) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

C1orf35 Antibody in Western Blot (WB) — Western blot analysis of C1orf35 in extracts from various samples. Samples were incubated with C1orf35 polyclonal antibody (Product # PA5-99222). Lane 1: Mouse spleen, blocked with antigen-specific peptides; Lane 2: Mouse spleen; Lane 3: Mcf7 cells (serum starvation treatment); Lane 4: P19 cells (UV treatment).

Immunogen image

C1orf35 Polyclonal Antibody

Immunogen Image C1orf35 Polyclonal Antibody

产品信息

PA5-99222

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human MMTAG2, corresponding to a region within C-terminal amino acids.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total MMTAG2.

靶标信息

MMTAG2 gene ontology (GO) annotations include neutrophil degranulation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: hMMTAG2; multiple myeloma transforming 2; Multiple myeloma tumor-associated protein 2; Multiple myeloma tumor-associated protein 2 homolog

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基因别名: C1orf35; MMTAG2

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UniProt ID: (Mouse) Q99LX5, (Human) Q9BU76

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Entrez Gene ID: (Mouse) 67862, (Human) 79169

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poly(A) RNA binding protein binding

biological_process