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Bethyl Laboratories
CAD Polyclonal Antibody
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FIGURE: 1 / 3
CAD Antibody (IHC-00280) in IHC (P)
Detection of human CAD by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CAD (Product # IHC-00280) used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed IHC Antibody Hilyte Plus™ 555 used at a dilution of 1:100.
Product Details
IHC-00280
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1650 and 1700 of human carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Dog, Rabbit, Guinea pig_10141, Pig, Orangutan, Monkey, Gorilla, Chimpanzee, Black rat and Gray short-tailed opossum
Target Information
The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CAD trifunctional protein; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; Multifunctional protein CAD
Gene Aliases: CAD; CDG1Z; DEE50; EIEE50; GATD4
UniProt ID: (Human) P27708
Entrez Gene ID: (Human) 790
nucleotide binding
catalytic activity
aspartate carbamoyltransferase activity
carbamoyl-phosphate synthase (ammonia) activity
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
dihydroorotase activity
glutaminase activity
protein kinase activity
protein binding
ATP binding
zinc ion binding
amino acid binding
transferase activity
carboxyl- or carbamoyltransferase activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
ligase activity
enzyme binding
identical protein binding
metal ion binding
aspartate binding
liver development
'de novo' pyrimidine nucleobase biosynthetic process
pyrimidine nucleotide biosynthetic process
UDP biosynthetic process
UTP biosynthetic process
cellular amino acid metabolic process
glutamine metabolic process
phosphate-containing compound metabolic process
xenobiotic metabolic process
heart development
female pregnancy
lactation
response to amine
citrulline biosynthetic process
response to caffeine
organ regeneration
response to insulin
response to testosterone
response to starvation
'de novo' UMP biosynthetic process
response to cortisol
nucleobase-containing small molecule metabolic process
cellular response to epidermal growth factor stimulus
cellular response to xenobiotic stimulus
pyrimidine-containing compound biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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