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Invitrogen
CASC5 Polyclonal Antibody
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CASC5 Antibody (PA5-114943) in ICC/IF
Immunocytochemistry analysis of CASC5 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114943) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-114943
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
C. elegans
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human KNL1(Accession Q8NG31), corresponding to amino acid residues N1621-V1671.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total CASC5.
靶标信息
Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Required for attachment of the kinetochores to the spindle microtubules. Directly links BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: AF15q14; ALL1-fused gene from chromosome 15q14 protein; Blinkin; blinkin, bub-linking kinetochore protein; Bub-linking kinetochore protein; cancer susceptibility candidate 5; Cancer susceptibility candidate gene 5 protein; Cancer susceptibility candidate gene 5 protein homolog; Cancer/testis antigen 29; CT29; kinetochore null 1 homolog; Kinetochore scaffold 1; Kinetochore-null protein 1; microcephaly, primary autosomal recessive 4; Outer kinetochore KNL1 complex subunit KNL1; Protein CASC5; Protein D40/AF15q14; protein phosphatase 1, regulatory subunit 55; RAD51 homolog
基因别名: 2310043D08Rik; 5730505K17Rik; AF15Q14; CASC5; CT29; D40; hKNL-1; hSpc105; KIAA1570; KNL1; MCPH4; PPP1R55; Rad51; Spc7
UniProt ID: (Mouse) Q66JQ7, (Human) Q8NG31
Entrez Gene ID: (Mouse) 76464, (Rat) 311327, (Human) 57082
acrosome assembly
sister chromatid cohesion
mitotic nuclear division
attachment of spindle microtubules to kinetochore
negative regulation of phosphatase activity
CENP-A containing nucleosome assembly
protein localization to kinetochore
cell division
spindle assembly checkpoint
cell cycle
chromosome segregation
biological_process
