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Agrisera
CCA1/Circadian clock associated 1 Polyclonal Antibody
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CCA1/Circadian clock associated 1 Antibody (AS13-2659) in WB
A clear band for CCA 1 was detected at about 80 k Da after electrophoresis using a 8% polyacrylamide gel cast in a Bio-Rad gel device with 15 wells and 1 mm thickness. Nuclear extract of Arabidopsis thaliana Ws (Wasilewska), zt 0 zt 6 and lhy/cca 1/toc 1 zt 0 was analyzed and protein concentration was equalized according to Bradford quantification to ~10 µg proteins per lane after resuspending and heating t... View More
产品信息
AS13-2659
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCA1 protein sequence.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
CCA1 is a protein-coding locus whose product is a MYB-related, sequence-specific DNA-binding transcription factor that localizes to the nucleus; in Arabidopsis, the gene comprises 8 exons and produces transcripts encoding a protein with an N-terminal single MYB-like DNA-binding region required for promoter recognition. Functionally, CCA1 is a core component of the plant circadian oscillator: its expression is light responsive and circadian regulated, and the protein participates in a feedback loop by binding the evening element in clock gene promoters (including TOC1) to repress transcription, thereby helping generate and phase daily rhythms in downstream gene expression. Genetic and biochemical data indicate CCA1 and the related clock factor LHY occupy the same promoter regions, can colocalize in the nucleus, and physically interact (including heterodimerization) to act synergistically in maintaining normal circadian period and regulating clock outputs such as photomorphogenesis and flowering time.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT2G46830; AtCCA1; circadian clock associated 1; F19D11.11; MYB-RELATED DNA BINDING PROTEIN
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