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Invitrogen
CCDC36 Polyclonal Antibody
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CCDC36 Antibody (PA5-114403) in ICC/IF
Immunocytochemistry analysis of CCDC36 in SiHa cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in CCDC36 polyclonal antibody (Product # PA5-114403) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-rabbit IgG (30 min, 37°C) and DAPI at a dilution of 1:100.
产品信息
PA5-114403
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human CCDC36 recombinant protein (Position: E172-N472).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, human HL-60 whole cell. IHC: human testis cancer tissue, human melanoma tissue. ICC/IF: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cancer/testis antigen 74; Coiled-coil domain-containing protein 36; CT74; Interactor of HORMAD1 protein 1
基因别名: CCDC36; CT74; IHO1
UniProt ID: (Human) Q8IYA8
Entrez Gene ID: (Human) 339834
