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Invitrogen
CCL14 Polyclonal Antibody, PeproTech®
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CCL14 Antibody (500-P106-1MG) in ELISA
Sandwich ELISA: To detect Human HCC-1 (CCL14) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CCL14 Polyclonal Antibody (Product # 500-P106-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CCL14 Polyclonal Antibody, Biotin (Product # 500-P106BT-500UG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human HCC-1 (CCL14).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
500-P106-1MG
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human HCC-1 (CCL14) (72 a.a.)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
0.1-1.0 mg/mL
规格
1 mg
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
产品详细信息
AA Sequence of recombinant protein: TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human HCC-1 (CCL14). Anti-Human HCC-1 (CCL14)-specific antibody was purified by affinity chromatography employing an immobilized Human HCC-1 (CCL14) matrix.
Sandwich ELISA: To detect Human HCC-1 (CCL14) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human HCC-1 (CCL14) (500-P106Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human HCC-1 (CCL14).
Western Blot: To detect Human HCC-1 (CCL14) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human HCC-1 (CCL14) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
This gene, CCL14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. This gene expresses both monocistronic and bicistronic transcripts. Bicistronic transcripts include the upstream cytokine gene CCL15. In addition, CCL14 undergoes alternative splicing in the bicistronic transcripts; it is unknown if its monocistronic transcript is also subject to alternative splicing.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: C-C motif chemokine 14; Chemokine CC-1/CC-3; H HCC-1(1-74); HCC-1(1-74); HCC-1/HCC-3; NCC-2; Small-inducible cytokine A14
基因别名: CCL14; NCC2; SCYA14
Entrez Gene ID: (Human) 6358
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