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Invitrogen
CCL5 (RANTES) Polyclonal Antibody, PeproTech®
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CCL5 (RANTES) Antibody (500-P118-1MG) in ELISA
Sandwich ELISA: To detect Murine RANTES (CCL5) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CCL5 (RANTES) Polyclonal Antibody (Product # 500-P118-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CCL5 (RANTES) Polyclonal Antibody, Biotin (Product # 500-P118BT-500UG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine RANTES (CCL5).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
500-P118-1MG
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse,
Rat
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Murine RANTES (CCL5)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
0.1-1.0 mg/mL
规格
1 mg
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
产品详细信息
AA Sequence of recombinant protein: SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine RANTES (CCL5). Anti-Murine RANTES (CCL5)-specific antibody was purified by affinity chromatography employing an immobilized Murine RANTES (CCL5) matrix.
Sandwich ELISA: To detect Murine RANTES (CCL5) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine RANTES (CCL5) (500-P118Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine RANTES (CCL5).
Western Blot: To detect Murine RANTES (CCL5) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine RANTES (CCL5) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Ccl5; chemokine (C-C motif) ligand 5; MGC17164; monocyte chemoattractant cytokine; OTTHUMP00000197106; RANTES; SIS δ; SIS-delta; SISδ; small-inducible cytokine A5; T-cell-specific protein RANTES; unnamed protein product
基因别名: MuRantes; RANTES; Scya5; SISd; TCP228
UniProt ID: (Mouse) P30882
Entrez Gene ID: (Mouse) 20304
phosphatidylinositol phospholipase C activity
protein kinase activity
chemokine activity
heparin binding
phospholipase activator activity
receptor signaling protein tyrosine kinase activator activity
CCR1 chemokine receptor binding
CCR5 chemokine receptor binding
chemoattractant activity
chemokine receptor binding
identical protein binding
protein homodimerization activity
chemokine receptor antagonist activity
CCR chemokine receptor binding
positive regulation of defense response to virus by host
calcium ion transport
cellular calcium ion homeostasis
exocytosis
inflammatory response
immune response
leukocyte cell-cell adhesion
G-protein coupled receptor signaling pathway
cell-cell signaling
response to toxic substance
positive regulation of gene expression
positive regulation of macrophage chemotaxis
positive regulation of T cell chemotaxis
positive regulation of smooth muscle cell migration
positive regulation of cell migration
pseudopodium assembly
phospholipase D-activating G-protein coupled receptor signaling pathway
positive regulation of fever generation
positive regulation of TOR signaling
positive regulation of cell-cell adhesion mediated by integrin
response to cytokine
positive regulation of homotypic cell-cell adhesion
response to interferon-gamma
response to tumor necrosis factor
T-helper 1 cell diapedesis
chemokine (C-C motif) ligand 5 signaling pathway
positive regulation of T cell proliferation
neutrophil activation
regulation of programmed cell death
negative regulation by host of viral transcription
cellular response to fibroblast growth factor stimulus
negative regulation of viral genome replication
positive regulation of neuron differentiation
positive regulation of osteoclast differentiation
negative regulation of G-protein coupled receptor protein signaling pathway
positive regulation of G-protein coupled receptor protein signaling pathway
positive regulation of angiogenesis
positive regulation of cell adhesion
eosinophil chemotaxis
lymphocyte chemotaxis
positive regulation of smooth muscle cell proliferation
positive regulation of epithelial cell proliferation
regulation of insulin secretion
regulation of T cell activation
positive chemotaxis
positive regulation of protein kinase B signaling
positive regulation of calcium ion transport
positive regulation of mast cell chemotaxis
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
negative regulation of chemokine-mediated signaling pathway
negative regulation of T cell apoptotic process
positive regulation of T cell apoptotic process
ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
positive regulation of monocyte chemotaxis
cellular response to virus
positive regulation of neuroinflammatory response
positive regulation of STAT cascade
negative regulation of macrophage apoptotic process
positive regulation of chemokine (C-X-C motif) ligand 2 production
positive regulation of T cell migration
positive regulation of natural killer cell chemotaxis
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