Invitrogen

CCL5 (RANTES) Polyclonal Antibody, Biotin, PeproTech®

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CCL5 (RANTES) Polyclonal Antibody, Biotin, PeproTech®

产品信息

500-P78BT-25UG

应用
建议稀释比
已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL
-

酶联免疫吸附实验 (ELISA)

0.25-1.0 µg/mL
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产品规格

种属反应

Rat

已发表种属

Rat

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived, 7.9 kDa Recombinant Rat RANTES (CCL5)
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偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2930765

产品详细信息

AA Sequence of recombinant protein: SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat RANTES (CCL5). Anti-Rat RANTES (CCL5)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Rat RANTES by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat RANTES (500-P78) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat RANTES.

Western Blot: To detect Rat RANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat RANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P78BT-1MG will be provided as 2 x 500 µg

靶标信息

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: C-C motif chemokine 5; MGC17164; OTTHUMP00000197106; regulated upon activation normal T-cell expressed and secreted; SIS δ; SIS-delta; SISδ; small inducible cytokine A5; small-inducible cytokine A5; T-cell-specific protein RANTES

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基因别名: Rantes; Scya5

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Entrez Gene ID: (Rat) 81780

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功能
phosphatidylinositol phospholipase C activity protein kinase activity chemokine activity heparin binding phospholipase activator activity receptor signaling protein tyrosine kinase activator activity CCR1 chemokine receptor binding CCR5 chemokine receptor binding chemoattractant activity chemokine receptor binding protein homodimerization activity protein self-association chemokine receptor antagonist activity cytokine intercellular signal molecule
参与通路
MAPK cascade chronic inflammatory response protein phosphorylation calcium ion transport cellular calcium ion homeostasis exocytosis inflammatory response leukocyte cell-cell adhesion G-protein coupled receptor signaling pathway cell-cell signaling aging response to virus response to bacterium response to toxic substance response to salt stress positive regulation of gene expression positive regulation of macrophage chemotaxis positive regulation of T cell chemotaxis positive regulation of phosphatidylinositol 3-kinase signaling response to organic cyclic compound response to activity positive regulation of smooth muscle cell migration dibenzo-p-dioxin metabolic process positive regulation of cell migration pseudopodium assembly positive regulation of cellular biosynthetic process activation of phospholipase D activity positive regulation of fever generation lipopolysaccharide-mediated signaling pathway response to nutrient levels response to lipopolysaccharide response to insulin positive regulation of cell-cell adhesion mediated by integrin response to cytokine positive regulation of homotypic cell-cell adhesion response to interferon-gamma response to tumor necrosis factor T-helper 1 cell diapedesis positive regulation of T cell proliferation neutrophil activation positive regulation of phosphorylation response to drug positive regulation of tyrosine phosphorylation of STAT protein protein kinase B signaling cellular protein complex assembly response to estrogen negative regulation by host of viral transcription cellular response to fibroblast growth factor stimulus negative regulation of viral genome replication positive regulation of neuron differentiation positive regulation of osteoclast differentiation negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of angiogenesis positive regulation of cell adhesion eosinophil chemotaxis lymphocyte chemotaxis positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation regulation of insulin secretion regulation of T cell activation positive chemotaxis protein tetramerization response to glucocorticoid positive regulation of calcium ion transport negative regulation of cell death positive regulation of mast cell chemotaxis positive regulation of protein tyrosine kinase activity chemokine-mediated signaling pathway negative regulation of chemokine-mediated signaling pathway negative regulation of T cell apoptotic process positive regulation of T cell apoptotic process response to cholesterol cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to vitamin K cellular response to morphine cellular response to cytokine stimulus cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to ethanol cellular response to lipid cellular response to high density lipoprotein particle stimulus cellular response to organic cyclic compound cellular response to alkyl hydroperoxide cellular response to transforming growth factor beta stimulus positive regulation of monocyte chemotaxis regulation of neuron death negative regulation of neuron death negative regulation of macrophage apoptotic process positive regulation of T cell migration positive regulation of natural killer cell chemotaxis
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