Invitrogen

CCNA1 Polyclonal Antibody

Name: CCNA1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-114439

查看其他18个Cyclin A1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

CCNA1 Antibody in Immunocytochemistry (ICC/IF)

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CCNA1 Antibody (PA5-114439) in ICC/IF

Immunocytochemistry analysis of Cyclin A1/CCNA1 using anti-Cyclin A1/CCNA1 antibody (Product # PA5-114439) . Cyclin A1/CCNA1 was detected in a section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-Cyclin A1/CCNA1 antibody (Product # PA5-114439) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. T... View More

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Immunogen Image CCNA1 Polyclonal Antibody

产品信息

PA5-114439

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Cyclin A1 recombinant protein (Position: D64-E263).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2884869

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human placenta tissue, human U937 whole cell, human PC-3 whole cell, human THP-1 whole cell, human MCF-7 whole cell, human Hela whole cell. ICC/IF: PC-3 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's). They are expressed temporally to control transition at different specific phases of the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。