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Invitrogen
CD100 Polyclonal Antibody
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FIGURE: 1 / 6
CD100 Antibody (PA5-47711) in IHC (F)
Immunohistochemical analysis of CD100 in immersion fixed frozen sections of mouse embryonic eye cup (15 d.p.c.). Samples were incubated in CD100 polyclonal antibody (Product # PA5-47711) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm.
Product Details
PA5-47711
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4D/CD100 Phe24-Thr657
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) Semaphorin 3A, rhSemaphorin 4B, and rhSemaphrin 6C is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD100; M-Sema G; Sema J; semaphorin H; Semaphorin-4D; Semaphorin-C-like 2; Semaphorin-J
Gene Aliases: CD100; coll-4; Sema4d; Semacl2; Semaj; Semcl2
UniProt ID: (Mouse) O09126
Entrez Gene ID: (Mouse) 20354
negative regulation of transcription from RNA polymerase II promoter
positive regulation of protein phosphorylation
negative regulation of cell adhesion
multicellular organism development
nervous system development
regulation of cell shape
negative regulation of alkaline phosphatase activity
positive regulation of phosphatidylinositol 3-kinase signaling
cell differentiation
positive regulation of cell migration
regulation of cell projection organization
positive regulation of GTPase activity
ossification involved in bone maturation
negative regulation of osteoblast differentiation
positive regulation of collateral sprouting
regulation of dendrite morphogenesis
positive regulation of peptidyl-tyrosine phosphorylation
negative regulation of peptidyl-tyrosine phosphorylation
positive regulation of axonogenesis
leukocyte aggregation
semaphorin-plexin signaling pathway
semaphorin-plexin signaling pathway involved in bone trabecula morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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