Invitrogen

CD102 (ICAM-2) Monoclonal Antibody (CBR-IC2/2), eBioscience™

Name: CD102 (ICAM-2) Monoclonal Antibody (CBR-IC2\/2), eBioscience\u2122
Brand: Invitrogen
SKU: 14-1029-82

2 篇参考文献

查看其他34个ICAM-2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

CD102 (ICAM-2) Antibody in Immunocytochemistry (ICC/IF)

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CD102 (ICAM-2) Antibody (14-1029-82) in ICC/IF

Immunofluorescence analysis of CD102 (ICAM-2) was performed using log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD102 (ICAM-2) Mouse Monoclonal Antibody (CBR-IC2/2) (Product # 14-1029-82) at 5 µg/mL in 0.1% BSA, incubated at 4 degre... View More

CD102 (ICAM-2) Antibody in Flow Cytometry (Flow)

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产品信息

14-1029-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

免疫组化 (IHC)

Assay-Dependent

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免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

0.125 µg/test

免疫沉淀 (IP)

Assay-Dependent

中和实验 (Neu)

查看1篇已发表文章 1篇已发表文章

功能检测 (Functional)

Assay-Dependent

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

CBR-IC2/2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_953553

产品详细信息

Description: The CBRIC2/2 monoclonal antibody reacts with human ICAM-2 (CD102). ICAM-2 is a 55-65 kD transmembrane glycoprotein possessing 2 extracellular Ig domains, a single transmembrane domain, and a short 26-amino acid cytoplasmic domain. ICAM-2 is expressed on most leukocytes, and is strongly expressed on vascular endothelial cells. ICAM-2 is a ligand of integrin CD11a/CD18 (LFA-1), and also has been reported to bind integrin CD11b/CD18 (Mac-1). ICAM-2 is an intercellular adhesion molecule involved in lymphocyte homing to sites of inflammation. The CBRIC2/2 antibody has been reported to have blocking activity.

Applications Reported: This CBRIC2/2 antibody has been reported for use in flow cytometric analysis, immunoblotting, immunoprecipitation, immunohistochemical staining, and functional assays.

Applications Tested: This CBRIC2/2 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The CBRIC2/2 antibody has also been found useful for immunoblotting of a human peripheral blood cell lysate, recognizing a protein of approximately 55 kD under non-reducing conditions. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ICAM-2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. ICAM-2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene. Diseases associated with ICAM2 include Colon Carcinoma In Situ.

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