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Invitrogen
CD106 (VCAM-1) Monoclonal Antibody (429), PE-Cyanine7, eBioscience™
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CD106 (VCAM-1) Antibody (25-1061-82) in Flow
C57BL/6 mouse bone marrow cells were stained with F4/80 Monoclonal Antibody, APC (Product # 17-4801-82) and 0.125 µg of Rat IgG2a kappa Isotype Control, PE-Cyanine7 (Product # 25-4321-82) (left) or 0.125 µg of CD106 Monoclonal Antibody, PE-Cyanine7 (right). Cells in the large scatter population were used for analysis.
产品信息
25-1061-82
产品规格
种属反应
Mouse
已发表种属
Not Applicable
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
429
偶联物
激发/发射光谱
569/780 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2784660
产品详细信息
Description: The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin a4b7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.
Applications Reported: This 429 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 429 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
靶标信息
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: DKFZp779G2333; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; vascular cell adhesion protein 1
基因别名: CD106; Vcam-1
Entrez Gene ID: (Mouse) 22329
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell-matrix adhesion
heart development
amine metabolic process
viral process
single organismal cell-cell adhesion
positive regulation of T cell proliferation
leukocyte tethering or rolling
oxidation-reduction process
cell chemotaxis
embryonic placenta morphogenesis
chorio-allantoic fusion
visceral serous pericardium development
cellular response to glucose stimulus
