Invitrogen

CD115 (c-fms) Monoclonal Antibody (12-3A3-1B10), eBioscience™

Name: CD115 (c-fms) Monoclonal Antibody (12-3A3-1B10), eBioscience\u2122
Brand: Invitrogen
SKU: 14-1159-82

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说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
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CD115 (c-fms) Antibody in Flow Cytometry (Flow)

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CD115 (c-fms) Antibody (14-1159-82) in Flow

Staining of normal human peripheral blood cells with 0.5 µg of Rat IgG2a K Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.5 µg of Anti-Human CD115 (c-fms) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Cells in the monocyte gate were used for analysis.

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产品信息

14-1159-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

免疫组化 (IHC)

Assay-Dependent

流式细胞分析 (Flow)

1 µg/test

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免疫沉淀 (IP)

Assay-Dependent

产品规格

种属反应

Human

已发表种属

C. elegans, Human

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

12-3A3-1B10

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_493929

产品详细信息

Description: CD115 or Colony-Stimulating Factor-1 Receptor (CSF-1R) is expressed on cells committed to the monocyte lineage and osteoclasts. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subsequently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. CSF-1R activation promotes the survival, proliferation and differentiation of mononuclear phagocytes and the spreading and motility of macrophages. In osteoclasts, CSF-1R synergizes with RANKL to regulate the differentiation of mononuclear phagocytes to osteoclasts.

Applications Reported: This 12-3A3-1B10 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested: This 12-3A3-1B10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: CD115; CD115 antigen; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; FMS proto-oncogene; kinase CSFR; macrophage colony stimulating factor I receptor; Macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; OTTHUMP00000224093; Proto-oncogene c-Fms

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基因别名: C-FMS; CD115; CSF-1R; CSF1R; CSFR; FIM2; FMS; HDLS; M-CSF-R

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UniProt ID: (Human) P07333

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Entrez Gene ID: (Human) 1436

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功能

macrophage colony-stimulating factor receptor activity ATP binding protein phosphatase binding cytokine binding protein homodimerization activity transmembrane signal receptor

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