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Invitrogen
CD133 Monoclonal Antibody (5E3)
高级验证
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
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CD133 Antibody (MA5-18323) in WB
Western Blot was performed using Anti-CD133 Monoclonal Antibody (5E3) (Product # MA5-18323) and a 133 kDa band corresponding to Prominin-1 was observed across cell lines tested except HeLa and SK-O-V3 which are reported to be low or negative for CD133 expression. Membrane enriched extracts (30 µg lysate) of NTERA-2 cl.D1 (Lane 1), Caco-2 (Lane 2), HT-29 (Lane 3), HeLa (Lane 4) and SK-O-V3 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulo... View More
产品信息
MA5-18323
产品规格
种属反应
Human
已发表种属
Mouse
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
5E3
抗原
Recombinant full length human CD133
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2539686
产品详细信息
This antibody detects endogenous protein at a molecular weight of 133 kDa and does not cross-react with related proteins.
Purity is >95% by SDS-PAGE.
靶标信息
The CD133 (AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. CD133 is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. CD133 has been shown to be expressed in cancers, including some leukemias and brain tumors, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of the CD133 gene is also associated with several types of cancer. CD133 is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner, and multiple transcript variants encoding different isoforms have been found.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Antigen AC133; antigen CD133; CD133; hematopoietic stem cell antigen; hProminin; Lamp II; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; Mac3; Prominin-1; Prominin-like protein 1; Prominin1
基因别名: AC133; CD133; CORD12; MCDR2; MSTP061; PROM1; PROML1; RP41; STGD4
UniProt ID: (Human) O43490
Entrez Gene ID: (Human) 8842
retina layer formation
photoreceptor cell maintenance
retina morphogenesis in camera-type eye
camera-type eye photoreceptor cell differentiation
glomerular visceral epithelial cell differentiation
glomerular parietal epithelial cell differentiation
positive regulation of nephron tubule epithelial cell differentiation
