Invitrogen

CD150 Monoclonal Antibody (A12 (7D4)), FITC, eBioscience™

Name: CD150 Monoclonal Antibody (A12 (7D4)), FITC, eBioscience\u2122
Brand: Invitrogen
SKU: 11-1509-42

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1 篇参考文献

查看其他40个CD150抗体

说明书

实验方案

问题与答案

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说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
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CD150 Antibody (11-1509-42) in Flow

Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 APC, CD19 PE-Cy7 and CD150 FITC. As expected based on known relative expression patterns, CD150 clone A12 (7D4) stains monocytes (orange), granulocytes (blue) and a subset of lymphocytes (pink).

Product Image Gallery

CD150 Antibody — Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 APC, CD19 PE-Cy7 and CD150 FITC. As expected based on known relative expression patterns, CD150 clone A12 (7D4) stains monocytes (orange), granulocytes (blue) and a subset of lymphocytes (pink). {RE}

产品信息

11-1509-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (1 µg)/test

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

A12 (7D4)

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10669579

产品详细信息

Description: The A12 monoclonal antibody reacts with human CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM induces proliferation and cytokine production of T cells and SLAM plays a role in activation of T and B cells.

Applications Reported: This A12 (7D4) antibody has been reported for use in flow cytometric analysis.

Applications Tested: The A12 (7D4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg)/test per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD150, also known as SLAM (Signaling Lymphocyte Activation Molecule), is a member of the immunoglobulin gene superfamily and plays a crucial role in T cell stimulation and immune cell interactions. It is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells. CD150 expression is rapidly upregulated upon activation, particularly on naive T cells, Th1 cells, and activated B cells, which express the membrane-bound, soluble, and cytoplasmic isoforms of SLAM. In T cells, signaling through SLAM induces proliferation and enhances the interferon-gamma response. SLAM is also involved in adhesion between T cells and antigen-presenting cells, facilitating immune cell interactions. Homophilic SLAM-SLAM binding during B to B and B to T cell interactions is suggested to enhance the expansion and differentiation of activated B cells. CD150 serves as a receptor for the measles virus and acts as a co-activator on T and B cells. It interacts with SH2D1A and PTPN11 via its cytoplasmic domain, playing a role in signal transduction. Mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP), highlighting its importance in immune regulation and disease pathology.

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