Invitrogen

CD150 Monoclonal Antibody (SLAM.4), Alexa Fluor™ 647

Name: CD150 Monoclonal Antibody (SLAM.4), Alexa Fluor\u2122 647
Brand: Invitrogen
SKU: MA5-44145

查看其他40个CD150抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

图: 1 / 2

CD150 Antibody (MA5-44145) in Flow

Separation of human CD150 positive lymphocytes (red-filled) from CD150 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD150 (SLAM.4) Alexa Fluor® 647 Monoclonal antibody (Product # MA5-44145) using a dilution of 4 µL reagent/100 µL of peripheral whole blood.

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Immunogen image

Immunogen Image CD150 Monoclonal Antibody (SLAM.4), Alexa Fluor™ 647

产品信息

MA5-44145

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

Assay-dependent

流式细胞分析 (Flow)

4 µL/1x10^6 cells

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

SLAM.4

抗原

Human CD150-transfected 300.19 cells

查看免疫原

偶联物

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

激发/发射光谱

650/671 nm 查看光谱

形式

Liquid

纯化类型

Size-exclusion chromatography

保存液

PBS with 0.2% BSA

内含物

15mM sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2913077

产品详细信息

This antibody recognizes an extracellular epitope of CD150, a cell surface molecule expressed on lymphocytes and involved in their activation.

靶标信息

CD150, also known as SLAM (Signaling Lymphocyte Activation Molecule), is a member of the immunoglobulin gene superfamily and plays a crucial role in T cell stimulation and immune cell interactions. It is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells. CD150 expression is rapidly upregulated upon activation, particularly on naive T cells, Th1 cells, and activated B cells, which express the membrane-bound, soluble, and cytoplasmic isoforms of SLAM. In T cells, signaling through SLAM induces proliferation and enhances the interferon-gamma response. SLAM is also involved in adhesion between T cells and antigen-presenting cells, facilitating immune cell interactions. Homophilic SLAM-SLAM binding during B to B and B to T cell interactions is suggested to enhance the expansion and differentiation of activated B cells. CD150 serves as a receptor for the measles virus and acts as a co-activator on T and B cells. It interacts with SH2D1A and PTPN11 via its cytoplasmic domain, playing a role in signal transduction. Mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP), highlighting its importance in immune regulation and disease pathology.

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