Invitrogen

CD150 Recombinant Rat Monoclonal Antibody (mShad150)

Name: CD150 Recombinant Rat Monoclonal Antibody (mShad150)
Brand: Invitrogen
SKU: 740148T

高级验证

查看其他40个CD150抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
高级验证 (1)

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CD150 Antibody (740148T) in Flow

Flow cytometry analysis of CD150 Recombinant Rat Monoclonal Antibody (mShad150) (Product # 740148T) was performed using Mouse splenocytes. Mouse splenocytes were stained with CD3 Monoclonal Antibody (OKT3), eFluor™ 450, eBioscience™ (Product # 48-0037-42) and 0.0625 µg of CD150 Recombinant Rat Monoclonal Antibody (mShad150) (Product # 740148T) (right) or unstained (left), followed by Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (Product # A78947, 1:1000). Viable cells were used for analysis as determ... View More

Product Image Gallery

CD150 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target. CD150 Recombinant Rat Monoclonal Antibody (mShad150) (Product # 740148T) specifically stains CD3 positive (CD3+) subset of Mouse splenocytes (pink histogram) while negligible CD150 staining is seen in CD3 negative (CD3-) subset of Mouse splenocytes (blue histogram). {RE}

产品信息

740148T

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.0625 µg/test

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2b, lambda

Expression System

ExpiCHO

分类

Recombinant Monoclonal

类型

Antibody

克隆号

mShad150

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

产品详细信息

This is an engineered recombinant antibody that was developed to provide superior sensitivity without changing the paratope. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro.

The advantages of recombinant antibodies include better specificity, lot-to-lot consistency and improved signal-to-noise ratio.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

CD150, also known as SLAM (Signaling Lymphocyte Activation Molecule), is a member of the immunoglobulin gene superfamily and plays a crucial role in T cell stimulation and immune cell interactions. It is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells. CD150 expression is rapidly upregulated upon activation, particularly on naive T cells, Th1 cells, and activated B cells, which express the membrane-bound, soluble, and cytoplasmic isoforms of SLAM. In T cells, signaling through SLAM induces proliferation and enhances the interferon-gamma response. SLAM is also involved in adhesion between T cells and antigen-presenting cells, facilitating immune cell interactions. Homophilic SLAM-SLAM binding during B to B and B to T cell interactions is suggested to enhance the expansion and differentiation of activated B cells. CD150 serves as a receptor for the measles virus and acts as a co-activator on T and B cells. It interacts with SH2D1A and PTPN11 via its cytoplasmic domain, playing a role in signal transduction. Mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP), highlighting its importance in immune regulation and disease pathology.

仅用于科研。不用于诊断过程。未经明确授权不得转售。