Invitrogen

CD150 Recombinant Rat Monoclonal Antibody (mShad150), Alexa Fluor™ Plus 555

Name: CD150 Recombinant Rat Monoclonal Antibody (mShad150), Alexa Fluor\u2122 Plus 555
Brand: Invitrogen
SKU: 740148TP555

高级验证

查看其他44个CD150抗体

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)
高级验证 (1)

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CD150 Antibody (740148TP555) in Flow

Flow cytometry analysis of CD150 Recombinant Rat Monoclonal Antibody (mShad150), Alexa Fluor™ Plus 555 (Product # 740148TP555) was performed using mouse splenocytes. Mouse splenocytes were stained with CD19 Monoclonal Antibody (eBio1D3 (1D3)), eFluor™ 450, eBioscience™ (Product # 48-0193-82) and 0.125 µg of CD150 Recombinant Rat Monoclonal Antibody (mShad150), Alexa Fluor™ Plus 555 (Product # 740148TP555) (right dot plot) or left unstained (left dot plot). Viable cells were used for analysis, as determined by LIVE/DEAD™ Fixable Near IR (78... View More

Product Image Gallery

CD150 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target. CD150 Recombinant Rat Monoclonal Antibody (mShad150), Alexa Fluor™ Plus 555 (Product # 740148TP555) specifically stains CD19 positive (CD19+) subset of mouse splenocytes (pink histogram) while negligible CD150 staining is seen in CD19 negative (CD19-) subset of mouse splenocytes (blue histogram). {RE}

产品信息

740148TP555

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2b, lambda

Expression System

ExpiCHO

分类

Recombinant Monoclonal

类型

Antibody

克隆号

mShad150

偶联物

Alexa Fluor™ Plus 555 Alexa Fluor™ Plus 555 Alexa Fluor™ Plus 555

激发/发射光谱

558/572 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

50 µg

纯化类型

Affinity chromatography

保存液

proprietary buffer, pH 6.3-7.3

内含物

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Wet ice

产品详细信息

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD150, also known as SLAM (Signaling Lymphocyte Activation Molecule), is a member of the immunoglobulin gene superfamily and plays a crucial role in T cell stimulation and immune cell interactions. It is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells. CD150 expression is rapidly upregulated upon activation, particularly on naive T cells, Th1 cells, and activated B cells, which express the membrane-bound, soluble, and cytoplasmic isoforms of SLAM. In T cells, signaling through SLAM induces proliferation and enhances the interferon-gamma response. SLAM is also involved in adhesion between T cells and antigen-presenting cells, facilitating immune cell interactions. Homophilic SLAM-SLAM binding during B to B and B to T cell interactions is suggested to enhance the expansion and differentiation of activated B cells. CD150 serves as a receptor for the measles virus and acts as a co-activator on T and B cells. It interacts with SH2D1A and PTPN11 via its cytoplasmic domain, playing a role in signal transduction. Mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP), highlighting its importance in immune regulation and disease pathology.

仅用于科研。不用于诊断过程。未经明确授权不得转售。