Invitrogen

CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), eBioscience™

Name: CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), eBioscience\u2122
Brand: Invitrogen
SKU: 14-1522-82

13个已发表图片

41 篇参考文献

查看其他86个CTLA-4 (CD152)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

已发表图片 (13)

CD152 (CTLA-4) Antibody in Flow Cytometry (Flow)

图: 1 / 13

CD152 (CTLA-4) Antibody (14-1522-82) in Flow

Frontiers in immunology 2023 - Rig-like helicase (RLH) activation augments co-stimulation and inhibits CTLA-4, B7-1 and B7-2. WT mice were used to evaluate the effects of Rig-like helicase activation with Poly (I:C) on co-stimulation and the CTLA-4 axis. (A) RT-PCR was used to quantitate the levels of mRNA encoding ICOS, ICOS L and CD28 in the lungs from WT mice that received B16 cells (B16+) or vehicle control (B16-) and were treated wi... View More

CD152 (CTLA-4) Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

Product Image Gallery

产品信息

14-1522-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看39篇已发表文章 39篇已发表文章

免疫沉淀 (IP)

Assay-Dependent

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

UC10-4B9

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_467509

产品详细信息

Description: The UC10-4B9 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells at low level and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly.

Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.

Applications Reported: The UC10-4B9 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. It has also been reported in in vitro functional studies. (Please use Functional Grade purified UC10-4B9, Product # 16-1522, in functional assays).

Applications Tested: The UC10-4B9 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.

仅用于科研。不用于诊断过程。未经明确授权不得转售。