Invitrogen

CD154 (CD40 Ligand) Monoclonal Antibody (24-31), eFluor™ 450, eBioscience™

Name: CD154 (CD40 Ligand) Monoclonal Antibody (24-31), eFluor\u2122 450, eBioscience\u2122
Brand: Invitrogen
SKU: 48-1548-42

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说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
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CD154 (CD40 Ligand) Antibody in Flow Cytometry (Flow)

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CD154 (CD40 Ligand) Antibody (48-1548-42) in Flow

Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (500X) (Product # 00-4970-03) (right) and stained with Anti-Human CD8a PE (Product # 12-0088-80) and Anti-Human CD154 (CD40 Ligand) eFluor® 450. Total viable cells were used for analysis.

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产品信息

48-1548-42

应用

建议稀释比

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流式细胞分析 (Flow)

5 µL (0.25 µg)/test

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产品规格

种属反应

Cynomolgus monkey, Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

24-31

偶联物

eFluor™ 450 eFluor™ 450 eFluor™ 450

激发/发射光谱

405/445 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10717672

产品详细信息

Description: The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154.

Applications Reported: This 24-31 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 24-31 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CD154; CD40 antigen ligand; CD40 ligand; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-cell antigen Gp39; TNF-related activation protein; TRAP; tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5

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基因别名: CD154; CD40L; CD40LG; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP

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UniProt ID: (Human) P29965

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Entrez Gene ID: (Human) 959, (Cynomolgus monkey) 102138630

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功能

cytokine activity tumor necrosis factor receptor binding CD40 receptor binding protein binding

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