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Invitrogen
CD178 Polyclonal Antibody
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FIGURE: 1 / 8
CD178 Antibody (PA5-87385) in ICC/IF
Immunofluorescence analysis of FASLG (Tumor necrosis factor ligand superfamily member 6) was performed using 80% confluent log phase MCF7 and MCF 10A cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD178 Polyclonal Antibody (Product # PA5-87385) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secon... View More
Product Details
PA5-87385
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide
Conjugate
Form
Liquid
Concentration
1.46 mg/mL
Amount
146 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Mouse heart; Cellular Location: Cell membrane, Cytoplasmic vesicle lumen, Lysosome lumen, Nucleus, Single-pass type II membrane protein
Target Information
CD178 (Fas ligand, FasL) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor, FasR, is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. As a member of the TNF-cytokine family CD178 induces apoptosis when interacting with FasR. CD178 may exist as either membrane bound (45 kD) or soluble forms (26 kD). The soluble protein can be released from cells upon cleavage by metalloproteinases. Binding of CD178 to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins. CD178 is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye. CD178 expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney. It is assumed that induction of apoptosis through CD178 is predominantly involved in anti-viral immune responses. CD178 is a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis. Various cells express FAS, where CD178 is expressed predominantly on activated T cells. FAS and CD178 are involved in the down-regulation of immune reactions as well as T cell-mediated cytotoxicity. CD178 concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension. The Fas/ CD178 system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Apoptosis (APO 1) antigen ligand 1; apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APTL; CD178; CD178 antigen; CD95 ligand; CD95 ligand protein;Generalized lymphoproliferative disease (Gld); CD95-L; Fas antigen ligand; Fas ligand; Fas ligand (TNF superfamily, member 6); Fas-LG; generalized lymphoproliferative disease; mutant tumor necrosis factor family member 6; soluble form; TNFL; Tumor necrosis factor (ligand) superfamily member 6; Tumor necrosis factor ligand; tumor necrosis factor ligand 1A; Tumor necrosis factor ligand superfamily member 6; Tumor necrosis factor ligand superfamily member 6 (TNFL6 / TNFSF6); unnamed protein product
Gene Aliases: ALPS1B; APT1LG1; APTL; CD178; CD95-L; CD95L; Fas-L; FASL; FASLG; gld; TNFSF6; TNLG1A
UniProt ID: (Human) P48023, (Mouse) P41047
Entrez Gene ID: (Human) 356, (Mouse) 14103
negative regulation of transcription from RNA polymerase II promoter
apoptotic process
inflammatory cell apoptotic process
immune response
signal transduction
cell-cell signaling
positive regulation of cell proliferation
extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of angiogenesis
cellular chloride ion homeostasis
response to lipopolysaccharide
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of neuron apoptotic process
positive regulation of epidermal growth factor receptor signaling pathway
retinal cell programmed cell death
endosomal lumen acidification
T cell apoptotic process
necroptotic process
response to growth factor
cellular response to interferon-gamma
apoptotic signaling pathway
extrinsic apoptotic signaling pathway
necroptotic signaling pathway
release of sequestered calcium ion into cytosol by endoplasmic reticulum
positive regulation of phosphatidylserine exposure on apoptotic cell surface
positive regulation of endothelial cell apoptotic process
positive regulation of extrinsic apoptotic signaling pathway
transcription, DNA-templated
regulation of transcription, DNA-templated
activation of cysteine-type endopeptidase activity involved in apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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