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Invitrogen
CD178 (Fas Ligand) Monoclonal Antibody (14C2), eBioscience™
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产品信息
BMS199-2
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
14C2
抗原
Rec. Human Fas-L
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: BMS199/2 (14C2) specifically recognizes human Fas Ligand. Clone 14C2 is not yet reported to have a blocking function.
FasL is a member of the TNF family and is expressed by neutrophils, monocytes, and activated T cells and NK cells. The interaction of FasL with its receptor (CD95, Fas) induces Fas-mediated killing of lymphocytes. Human FasL is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. Therefore for optimal detection of surface FasL on activated peripheral blood cells, incubation of cells with an MMP inhibitor is recommended.
Purity is greater than 95% IgG1 as determined by SDS-PAGE.
The fluorochrome phycoerythrin (PE) shows an excitation maximum of 495 to 564 nm and an emission maximum of 576 nm. Annexin V conjugated to PE is suitable for the detection of PS and allows simple secondary labelling by staining, for example, the membrane surface proteins with a monoclonal antibody for further cellular characterization.
靶标信息
CD178 (Fas ligand, FasL) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor, FasR, is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. As a member of the TNF-cytokine family CD178 induces apoptosis when interacting with FasR. CD178 may exist as either membrane bound (45 kD) or soluble forms (26 kD). The soluble protein can be released from cells upon cleavage by metalloproteinases. Binding of CD178 to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins. CD178 is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye. CD178 expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney. It is assumed that induction of apoptosis through CD178 is predominantly involved in anti-viral immune responses. CD178 is a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis. Various cells express FAS, where CD178 is expressed predominantly on activated T cells. FAS and CD178 are involved in the down-regulation of immune reactions as well as T cell-mediated cytotoxicity. CD178 concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension. The Fas/ CD178 system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Apoptosis (APO 1) antigen ligand 1; apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APTL; CD178; CD178 antigen; CD95 ligand; CD95 ligand protein;Generalized lymphoproliferative disease (Gld); CD95-L; Fas antigen ligand; Fas ligand; Fas ligand (TNF superfamily, member 6); Fas-LG; mutant tumor necrosis factor family member 6; soluble form; TNFL; Tumor necrosis factor (ligand) superfamily member 6; Tumor necrosis factor ligand; tumor necrosis factor ligand 1A; Tumor necrosis factor ligand superfamily member 6; Tumor necrosis factor ligand superfamily member 6 (TNFL6 / TNFSF6)
基因别名: ALPS1B; APT1LG1; APTL; CD178; CD95-L; CD95L; FASL; FASLG; TNFSF6; TNLG1A
UniProt ID: (Human) P48023
Entrez Gene ID: (Human) 356
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
inflammatory cell apoptotic process
immune response
signal transduction
cell-cell signaling
positive regulation of cell proliferation
extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of angiogenesis
cellular chloride ion homeostasis
response to lipopolysaccharide
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of neuron apoptotic process
positive regulation of epidermal growth factor receptor signaling pathway
retinal cell programmed cell death
endosomal lumen acidification
T cell apoptotic process
necroptotic process
response to growth factor
apoptotic signaling pathway
extrinsic apoptotic signaling pathway
activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
necroptotic signaling pathway
regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of endothelial cell apoptotic process
