Invitrogen

CD19 Monoclonal Antibody (6OMP31), Alexa Fluor™ 488, eBioscience™

Name: CD19 Monoclonal Antibody (6OMP31), Alexa Fluor\u2122 488, eBioscience\u2122
Brand: Invitrogen
SKU: 53-0194-82

高级验证

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8 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

CD19 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD19 Antibody (53-0194-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 10 µg/mL Rat IgG2a K Isotype Control Alexa Fluor® 488 (left) or 10 µg/mL Anti-CD19 Alexa Fluor® 488 (right). Nuclei are counterstained with DAPI.

CD19 Antibody in Immunohistochemistry (IHC)

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CD19 Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of CD19 was observed in Raji, Ramos and Daudi which are reported to be positive and not in Jurkat, MOLT-4 and SW480 using CD19 Monoclonal Antibody (6OMP31), Alexa Fluor™ 488, eBioscience™ (Product # 53-0194-82) in western blot. {RE}

产品信息

53-0194-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5 µg/mL

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

10 µg/mL

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免疫组化（冰冻） (IHC (F))

10 µg/mL

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

6OMP31

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2637167

产品详细信息

Description: The 6OMP31 monoclonal antibody reacts with human, mouse, and rat CD19. CD19 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD19 was first identified as the B4 antigen of human B lymphocytes using the anti-B4 monoclonal. It is expressed by B cells during all stages of development, except on terminally differentiated plasma cells, and on follicular dendritic cells. CD19 acts primarily as a B cell co-receptor and together with CD21, CD81, Leu13, and MHC class II forms a multi-molecular complex that associates with the BCR. CD19 plays a role in the development, activation, differentiation, and proliferation of B cells. CD19 deficiency leads to an overall impaired humoral immune response. Mutations in CD19 have been associated with severe immunodeficiency syndromes. Although it is unclear whether CD19 contributes directly to B cell carcinogenesis, it is expressed on most B cell tumors, such as acute lymphoblastic leukemias and B cell lymphomas. The use of CD19 monoclonal antibodies for therapies against lymphoma, leukemia and autoimmune disorders are currently being explored.

Applications Reported: This 6OMP31 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This 6OMP31 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD19 is a member of the immunoglobulin superfamily, characterized by two Ig-like domains, and is expressed on B cells throughout all stages of development, excluding terminally differentiated plasma cells. It is also expressed on follicular dendritic cells and has been observed on myeloid leukemia cells, particularly those of monocytic lineage. CD19 is considered the earliest and broadest B cell-restricted antigen, and its expression is found in all B cell precursor leukemias. CD19 forms a multimolecular complex with CD21, CD81, Leu13, MHC class II, and the B cell receptor (BCR), playing a crucial role in B cell signaling. As a signal-amplifying coreceptor for the BCR, CD19 lowers the threshold for antigen receptor-dependent stimulation, allowing B cells to respond specifically and sensitively to various antigens through low-affinity antigen receptors. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. Beyond its role as a BCR coreceptor, CD19 can also signal independently of BCR co-ligation, serving as a central regulatory component upon which multiple signaling pathways converge. This makes CD19 an important functional regulator of both normal and malignant B cell proliferation. Mutations in the CD19 gene can result in hypogammaglobulinemia, a condition characterized by low levels of immunoglobulins, while CD19 overexpression can lead to B cell hyperactivity. CD19 is expressed on 100% of peripheral B cells, as defined by the expression of kappa or lambda light chains, underscoring its significance in B cell function and immune regulation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19; CD19 antigen; Differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12

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基因别名: AW495831; B4; CD19; CVID3

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UniProt ID: (Human) P15391, (Mouse) P25918

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Entrez Gene ID: (Human) 930, (Mouse) 12478, (Rat) 365367

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功能

receptor signaling protein activity protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity

参与通路

chromosome breakage B cell receptor signaling pathway positive regulation of release of sequestered calcium ion into cytosol cellular defense response cell surface receptor signaling pathway regulation of phosphatidylinositol 3-kinase signaling phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling regulation of immune response