Invitrogen

CD196 (CCR6) Monoclonal Antibody (sirx6), eFluor™ 660, eBioscience™

Name: CD196 (CCR6) Monoclonal Antibody (sirx6), eFluor\u2122 660, eBioscience\u2122
Brand: Invitrogen
SKU: 50-7196-82

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5 篇参考文献

查看其他32个CCR6抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (1)

CD196 (CCR6) Antibody in Flow Cytometry (Flow)

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CD196 (CCR6) Antibody (50-7196-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD19 PE (Product # 12-0193-82) and 0.5 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.5 µg of Anti-Mouse CD196 (CCR6) eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.

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Immunogen Image CD196 (CCR6) Monoclonal Antibody (sirx6), eFluor™ 660, eBioscience™

产品信息

50-7196-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

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其他PubMed (Misc)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

sirx6

抗原

N-terminal extracellular domain of mouse CCR6

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偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11219682

产品详细信息

Description: The sirx6 monoclonal antibody reacts with mouse CD196 (CCR6). CD196 is a seven transmembrane G protein-coupled receptor expressed on T, B, dendritic, NK and Langerhans cells. This CC chemokine receptor uniquely binds MIP3a/CCL20, a chemoattractant for dendritic cells, effector/memory T cells, and B cells. CD196 is also involved in host defense and inflammation at epithelial sites. Furthermore, this receptor has been implicated in Th17 differentiation and CD4+FoxP3+ regulatory T cell development.

Due to expression characteristics of some chemokine receptors (shedding, surface cleavage or intracellular cycling), we recommend a fixation step after surface staining. Refer to Applications Tested for more information.

Applications Reported: This sirx6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This sirx6 antibody has been tested by flow cytometric analysis of mouse splenocytes. Cells are surface stained, then washed with Flow Cytometry Staining Buffer, immediately fixed with IC Fixation Buffer for 20-30 minutes, and then washed with Flow Cytometry Staining Buffer prior to analysis. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD196, also known as CCR6, is a member of the beta chemokine receptor family and is characterized by a seven transmembrane structure similar to G protein-coupled receptors. This receptor uniquely binds to macrophage inflammatory protein 3 alpha (MIP-3alpha/CCL20), which acts as a chemoattractant for dendritic cells, effector/memory T cells, and B cells. CCR6 is preferentially expressed by immature dendritic cells and memory T cells, playing a crucial role in host defense and inflammation at epithelial sites. It is important for B-lineage maturation and antigen-driven B-cell differentiation, and it regulates the migration and recruitment of dendritic and T cells during inflammatory and immunological responses. Additionally, CCR6 has been implicated in the differentiation of Th17 cells and the development of CD4+FoxP3+ regulatory T cells, highlighting its role in immune regulation. Alternatively spliced transcript variants encoding the same protein have been described for this gene, further emphasizing its functional diversity in immune processes.

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