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CD199 (CCR9) Antibody (740187MP555) in ICC/IF
Immunofluorescence analysis of CD199 was performed using CD199 Recombinant Mouse Monoclonal Antibody (BBC3M4), Alexa Fluor™ Plus 555 (Product # 740187MP555) on 70-80% confluent MOLT-4 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, and then blocked with 2% BSA for 1 hour at room temperature. The cells were stained with CD199 Recombinant Mouse Monoclonal Antibody (BBC3M4), Alexa Fluor™ P... View More
产品信息
740187MP555
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
BBC3M4
偶联物
激发/发射光谱
558/572 nm
查看光谱
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein A/G
保存液
proprietary buffer, pH 6.8
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This BBC3M4 antibody has been tested by flow cytometric analysis of MOLT-4 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described.
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生物信息学
蛋白别名: C-C chemokine receptor type 9; C-C CKR-9; CDw199; CDw199 antigen; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; G-protein coupled receptor 28; GPR-9-6
基因别名: CC-CKR-9; CCR9; CDw199; GPR-9-6; GPR28
UniProt ID: (Human) P51686
Entrez Gene ID: (Human) 10803
