Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CD20 Monoclonal Antibody (AISB12), eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 4
CD20 Antibody (14-0201-82) in Flow
Staining of BALB/c splenocytes with Anti-Mouse CD19 Alexa Fluor® 647and 1.0 µg Anti-Mouse CD20 Purified followed by Anti-Rat IgG PE (Product # 12-4822). Quadrant lines represent Rat IgG2a isotype control staining levels and cells in the lymphocyte gate were used for analysis.
产品信息
14-0201-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
AISB12
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The AISB12 monoclonal antibody reacts with mouse CD20. CD20 is a B cell-specific 32kDa membrane protein involved in intracellular calcium regulation. CD20 expression begins after the induction of CD19 during development, and is coexpressed with IgM during the pre-B to immature B cell transition in bone marrow. Expression then increases during maturation with almost all mature cells expressing some level of CD20. The human CD20 antigen has proven to be an effective immunotherapeutic target in B cell lymphoma and several autoimmune diseases including rheumatoid arthritis and type I diabetes. CD20 consists of 4 transmembrane domains and a 44 amino acid extracellular loop domain where AISB12 binds.
Applications Reported: This AISB12 antibody has been reported for use in flow cytometric analysis and western blotting.
Applications Tested: This AISB12 antibody has been tested by immunoblot on A20 cell lysates and flow cytometry on mouse splenocytes.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: APY; ATOPY; B-cell differentiation antigen Ly-44; B-lymphocyte antigen CD20; CD20; CD20 antigen; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Ly44; Lymphocyte antigen 44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 2; MGC3969
基因别名: AA960661; Cd20; Ly-44; Ms4a1; Ms4a2
UniProt ID: (Mouse) P19437
Entrez Gene ID: (Rat) 309217, (Mouse) 12482
