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Invitrogen
CD200R3 Monoclonal Antibody (Ba103)
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产品信息
MA5-33337
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
Ba103
抗原
Mouse primary basophils; cell-enriched bone marrow cells
偶联物
形式
Liquid
浓度
100 µg/mL
纯化类型
Protein G
保存液
PBS with 0.1% BSA
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Product is supplied at 1 mL (100 µL/mL). This antibody recognizes mouse CD200 Receptor-like 3 (CD200R3), a protein of ~38 kDa or ~74 kDa (reduced or non-reduced condition, respectively).
Application Notes: Western Blot - Antibody was used in reducing and non-reducing conditions with MC/9 lysates. Under reducing conditions, the resulting band size was 38kDa, and under non-reducing conditions, the resulting band size was 74 kDa. Flow Cytometry - Basophils and mast cells were used. No fixation is needed. Functional Studies - Antibody was used as an agonist in basophil-mediated allergic reactions. A single intravenous administration of 30 µg antibody was used . The biological activity of the antibodyis a drastic reduction in the basophile number in the peripheral blood and spleen, to approximately 20% of the normal level. This product is also useful for mast cell degranulation. Readout was determined by measuring hexosaminidase activity. Immunoprecipitation - mast cells were lysed with 1% NP-40 containing lysis buffer with proteinase inhibitor mixture before incubation with the antibody.
靶标信息
CD200R3 is a dimeric cell surface receptor also known as CD200RLb. CD200R3 contains two Ig-like extracellular domains and as such is a member of the immunoglobulin superfamily of receptors. The intracellular region of CD200R3 associates with the adaptor molecule DAP12, and possibly also DAP10, CD3 zeta and FcR gamma-chain, indicating that it is capable of transducing stimulatory signals. In this respect CD200R3 bears similarity to activatory receptors CD200R2 and CD200R4. In contrast, an inhibitory receptor CD200R (also known as CD200R1) relies on other adaptors. CD200R3 is primarily expressed by basophils and mast cells, cells known to be involved in anti-parasitic immune responses and frequently also allergic reactions. The surface expression of CD200R3 decreases when basophils are activated by ligating their CD16 and CD32. Remarkably, most of this downregulation is observed within the first 30 minutes after activation. Unlike CD200R, CD200R3 does not bind CD200.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD200 cell surface glycoprotein receptor 3; CD200 cell surface glycoprotein receptor-like 3; CD200 cell surface glycoprotein receptor-like b; CD200 receptor-like 3; CD200RLb; Cell surface glycoprotein CD200 receptor 3; Cell surface glycoprotein OX2 receptor 3
基因别名: 4733401I18Rik; 4833409J19Rik; AI505817; BB106877; Cd200r3; Cd200rlb; mCD200RLb
UniProt ID: (Mouse) Q5UKY4
Entrez Gene ID: (Mouse) 74603
