Invitrogen

CD204 Monoclonal Antibody (M204PA), PE-Cyanine7, eBioscience™

Name: CD204 Monoclonal Antibody (M204PA), PE-Cyanine7, eBioscience\u2122
Brand: Invitrogen
SKU: 25-2046-82

查看其他31个CD204抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

图: 1 / 1

CD204 Antibody (25-2046-82) in Flow

Staining of BALB/c resident peritoneal exudate cells with Anti-Mouse F4/80 Antigen APC (Product # 17-4801-82) and 0.06 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.06 µg of Anti-Mouse CD204 PE-Cyanine7 (right). Total cells were used for analysis.

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产品信息

25-2046-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

- -

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

M204PA

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2637412

产品详细信息

This M204PA antibody recognizes mouse CD204 also known as Macrophage Scavenger Receptor 1 (MSR 1), Scavenger Receptor A (SR-A) or SCARA1. CD204 belongs to the class A family of scavenger receptors that are primarily responsible for binding a wide variety of macromolecules and are involved in many aspects of macrophage activity. Other members of this family include: MARCO (SCARA2), CSR (SCARA3), SRCL (SCARA4) and SCARA5. All class A scavenger receptors are trans-membrane proteins with extracellular C-termini and homotrimeric structures. CD204 exists as 2 splice variants: the more ubiquitous full length isoform (type I) and a shorter isoform (type II) that lacks the C-terminal cysteine-rich domain. Both types are functional and bind modified LDL with similar affinity. In addition to lipid metabolism, CD204 plays an important role suppressing inflammation and macrophage activation. CD204 is considered one of the markers of M2 and Tumor-Associated Macrophages. Interestingly, this molecule is responsible for adherence of RAW264.7 cells to tissue culture-treated plastic in the absence of Ca and Mg cations.

This M204PA antibody binds to CD204 of multiple mouse strains, including C57Bl/6, Balb/c and Swiss-Webster (outbred). This antibody works on fixed cells and can be used to stain CD204 intracellularly. The M204PA antibody does not cross-react with human or rat. This antibody is not suitable for IHC staining.

This M204PA antibody has been tested by flow cytometric analysis of mouse resident peritoneal exudate cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD204 gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. Diseases associated with MSR1 include Barrett Esophagus and Prostate Cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。