Invitrogen

CD206 (MMR) Monoclonal Antibody (MR6F3), Alexa Fluor™ 700, eBioscience™

Name: CD206 (MMR) Monoclonal Antibody (MR6F3), Alexa Fluor\u2122 700, eBioscience\u2122
Brand: Invitrogen
SKU: 56-2061-82

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说明书

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说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
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CD206 (MMR) Antibody in Flow Cytometry (Flow)

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CD206 (MMR) Antibody (56-2061-82) in Flow

BALB/c mouse resident peritoneal exudate cells were stained with F4/80 Monoclonal Antibody, FITC (Product # 11-4801-82) followed by fixation and permeabilization with the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00). Cells were then intracellularly stained with 0.125 µg of Rat IgG2b kappa Isotype Control, Alexa Fluor 700 (Product # 56-4031-80) (left) or 0.125 µg of CD206 (MMR) Monoclonal Antibody, Alexa Fluor 700 (right). Total viable cells were used for analysis.

CD206 (MMR) Antibody in Immunocytochemistry (ICC/IF)

CD206 (MMR) Antibody in Western Blot (WB)

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产品信息

56-2061-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Mouse

已发表种属

C. elegans

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

MR6F3

偶联物

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

激发/发射光谱

696/719 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2762723

产品详细信息

Description: This MR6F3 antibody recognizes mouse CD206 also known as Macrophage Mannose Receptor (MMR) or Mannose Receptor C, Type 1 (MRC1). CD206 is a 175-kDa, type 1 integral membrane glycoprotein receptor that is present in macrophages, some dendritic cells, as well as liver and lymphoid endothelial cells. CD206 belongs to the C-type lectin family. Extracellular regions of CD206 include an N-terminal cysteine-rich (CR) domain that binds sulfated glycoproteins, a fibronectin II (FNII) domain that binds collagens, and eight carbohydrate recognition domains (CRDs) that bind sugars like mannose and fucose with high affinity. CD206 mediates phagocytic and endocytic uptake of fungal, bacterial, protozoan and viral antigens, and plays an important role in immune defense and immune regulation. A soluble form of CD206 is generated by cleavage of the full-length protein, and it can be detected in in vitro macrophage cell culture supernatants and in mouse serum. CD206 is considered to be one of the markers of M2 macrophages. Factors inducing its expression include: IL-4, IL-13, M-CSF, IL-6, IL-10, and glucocorticoids, while TNF-alpha, IFN-gamma, TGF-beta, and LPS have been reported to down-regulate expression of CD206.

It has been demonstrated that only a small fraction of CD206 is present at the cell surface, therefore, intracellular staining is recommended.

Applications Reported: This MR6F3 antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis.

Applications Tested: This MR6F3 antibody has been tested by intracellular staining and flow cytometric analysis of mouse resident peritoneal exudate cells using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

靶标信息

CD206 (MSR, Mannose receptor, MRC1) is a 175 kDa transmembrane protein belonging to the group of pattern recognition receptors. CD206 is predominantly expressed in tissue macrophages, dendritic cells, a subpopulation of endothelial cells and sperm cells. CD206 is thought to play a role in the innate and adaptive immune response. CD206 is also expressed on microglia and mato cells of the brain but not astrocytes or neurons. CD206 also mediate the recognition and uptake of a variety of macromolecules, including modified lipoproteins, advanced glycation end (AGEs) products and amyloid b-protein (Abeta). While the normal role of CD206 is associated with cell adhesion and host defense mechanisms, it also has been implicated in the development of atherosclerosis and Amyloid beta deposition in Alzheimer's disease (AD). CD206's gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. CD206 has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. Other diseases associated with CD206 dysfunction include leprosy and Gaucher's Disease.

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