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Invitrogen
CD21 Monoclonal Antibody (HB5), PE-Cyanine5, eBioscience™
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CD21 Antibody (15-0219-42) in Flow
Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, FITC (Product # 11-0038-42) and Mouse IgG2a kappa Isotype Control, PE-Cyanine5 (Product # 15-4724-81) (left) or CD21 Monoclonal Antibody, PE-Cyanine5 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit (Product # L34962).
产品信息
15-0219-42
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
HB5
偶联物
激发/发射光谱
568/666 nm
查看光谱
形式
liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Description:
The HB5 monoclonal antibody reacts with human CD21, an approximately 145 kDa type I transmembrane protein. CD21 is expressed by mature B cells, folliculate dendritic cells and a subset of epithelial cells and is a receptor for complement component C3d and Epstein-Barr virus (EBV). CD21, in association with CD19 and CD81, participates in the multimolecular complex with BCR and is involved in B cell activation.
Applications Tested:
This HB5 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity:
This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
靶标信息
CD21 (complement receptor 2, CR2, C3D receptor, EBV receptor) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to follicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands. CD21 functions as receptor for C3d, C3dg and iC3b complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation. Genetic variations in the CD21 gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively, spliced transcript variants encoding different isoforms of CD21 have been found.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
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生物信息学
蛋白别名: CD21; Complement C3d receptor; Complement C3d receptor (C3DR); complement component (3d/Epstein Barr virus) receptor 2; Complement receptor type 2; Complement Receptor type 2 (CR2); Cr2; EBV receptor; EBV-R; Epstein-Barr virus receptor; EVBR
基因别名: C3DR; CD21; CR; CR2; CVID7; SLEB9
UniProt ID: (Human) P20023
Entrez Gene ID: (Human) 1380
