Invitrogen

CD217 (IL-17Ra) Monoclonal Antibody (424LTS), Functional Grade, eBioscience™

Name: CD217 (IL-17Ra) Monoclonal Antibody (424LTS), Functional Grade, eBioscience\u2122
Brand: Invitrogen
SKU: 16-7917-82

1 篇参考文献

查看其他29个IL17RA抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

CD217 (IL-17Ra) Antibody in Functional Assay (Functional)

图: 1 / 1

CD217 (IL-17Ra) Antibody (16-7917-82) in Functional

Neutralization of Human IL-17A Recombinant Protein activity by Anti-Human IL-17 Receptor A Functional Grade® Purified as measured by IL-6 induction in NHDF human fibroblast cells.

Product Image Gallery

产品信息

16-7917-82

应用

建议稀释比

已发表文章

中和实验 (Neu)

Assay-Dependent

功能检测 (Functional)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

424LTS

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10609205

产品详细信息

Description: The 424LTS monoclonal antibody reacts with and blocks IL-17A binding to human interleukin 17 receptor A (IL-17RA, IL-17R). IL-17RA is a type I membrane protein of approximately 96 kDa (866 amino acids) and is involved in inflammatory responses to IL-17A, IL-17F and IL-17AF heterodimer. It forms heterodimeric complexes with several other members of its receptor family, including IL-17RC for IL-17A and IL-17F signaling and IL-17RC for IL-17E (IL-25) signaling. IL-17RA is ubiquitously expressed, but in leukocytes its expression is elevated on granulocytes and monocytes.

Applications Reported: This 424LTS antibody has been reported for use in blocking IL-17 receptor A ligand binding.

Applications Tested: The ND50 of 424LTS as measured by the inhibition of IL-6 induction in NHDF cells is 1-5 µg/mL in the presence of 3 ng/mL of recombinant Human IL-17A. Neutralization dose will vary depending on assay method, cytokine concentration, and cell type. This antibody should be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

Interleukin 17A (IL-17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. IL-17RA is a ubiquitous type I membrane glycoprotein that binds with low affinity to IL-17A and IL-17F. IL-17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Defects in IL-17RA are the cause of familial candidiasis type 5 (CANDF5) which is a rare disorder with altered immune responses and impaired clearance of fungal infections.

仅用于科研。不用于诊断过程。未经明确授权不得转售。