Invitrogen

CD226 (DNAM-1) Monoclonal Antibody (10E5), Functional Grade, eBioscience™

Name: CD226 (DNAM-1) Monoclonal Antibody (10E5), Functional Grade, eBioscience\u2122
Brand: Invitrogen
SKU: 16-2261-81

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查看其他32个CD226 (DNAM-1)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
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CD226 (DNAM-1) Antibody in Flow Cytometry (Flow)

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CD226 (DNAM-1) Antibody (16-2261-81) in Flow

Staining of 3-day Anti-Mouse CD3/CD28-stimulated C57Bl/6 splenocytes with 0.25 µg of Rat IgG2b Kisotype Control Functional Grade Purified (Product # 16-4031-81) (left) or 0.25 µg of Anti-Mouse CD226 Functional Grade Purified (right) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87) and subsequently with Anti-Mouse CD8a FITC (Product # 11-0081-82).Total viable cells were used for analysis.

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产品信息

16-2261-81

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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功能检测 (Functional)

Assay-Dependent

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

10E5

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_1724031

产品详细信息

Description: This 10E5 monoclonal antibody reacts with mouse CD226, which is also known as DNAX accessory molecule-1 (DNAM-1). CD226 is an adhesion molecule expressed primarily on T cells, including thymocytes. The highest levels of CD226 are found on naive CD8 T cells. Moreover, expression of CD226 is regulated differentially in CD4 T cells. Under certain culture conditions, CD226 can be upregulated in Th1 and downregulated in Th2 and Th0 cells. This cell surface receptor can also be detected on natural killer cells, some B cell subsets, monocytes, and platelets. Interaction with its ligands, the poliovirus receptor (CD155) and CD112, results in adhesion and cell signaling events that promote innate and adaptive immunity, including differentiation, survival, and cytotoxicity.

The 10E5 monoclonal antibody has been reported to inhibit antigen-specific T cell proliferation and IFN-gamma production in vivo.

Applications Reported: This 10E5 antibody has been reported for use in flow cytometric analysis and functional assays.

Applications Tested: This 10E5 antibody has been tested by flow cytometric analysis of 3-day CD3/CD28-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

DNAM 1 is a receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes. DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells. DNAM-1 is also expressed by a subset of CD3 positive thymocytes.

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