Invitrogen

CD230 (PrP) Monoclonal Antibody (4D5), PE, eBioscience™

Name: CD230 (PrP) Monoclonal Antibody (4D5), PE, eBioscience\u2122
Brand: Invitrogen
SKU: 12-9230-42

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5 篇参考文献

查看其他31个PrP抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (3)

CD230 (PrP) Antibody in Flow Cytometry (Flow)

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CD230 (PrP) Antibody (12-9230-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (open histogram) or Anti-Human CD230 (PrP) PE (filled histogram). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-9230-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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产品规格

种属反应

Human

已发表种属

C. elegans, Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

4D5

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2572704

产品详细信息

Description: CD230 (prion protein; PrP^C) is a 35 kD glycoprotein, attached to the cell surface via a GPI-anchor. While the function of CD230 is unclear, prion protein has been the focus of research based on its involvement in transmissible spongiform encephalopathies (TSE), including Creutzfeld-Jakob disease in humans. CD230 undergoes a conformational change in which the cellular PrP^C form is converted to the scrapie PrP^Sc form. This conversion is essential for the infectiousness of prion-based diseases. CD230 is predominantly expressed on the surface of lymphocytes and monocytes, with weak expression on granulocytes and erythrocytes. In platelets, CD230 is expressed primarily intracellularly, and is upregulated to the surface upon activation.

Applications Reported: This 4D5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 4D5 antibody has been pre-titrated and tested. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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生物信息学

蛋白别名: Alternative prion protein; AltPrP; ASCR; BSE; CD230; CD230 antigen; Major prion protein; MGC26679; prion-related protein; PrP; PrP27-30; PrP33-35C; TSE

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基因别名: ALTPRP; ASCR; CD230; CJD; GSS; KURU; p27-30; PRIP; PRNP; PRP; PrP27-30; PrP33-35C; PrPc

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UniProt ID: (Human) F7VJQ1

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Entrez Gene ID: (Human) 5621

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功能

copper ion binding protein binding lamin binding microtubule binding tubulin binding identical protein binding ATP-dependent protein binding ion channel binding chaperone binding

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