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Invitrogen
CD244.2 (2B4) Monoclonal Antibody (eBio244F4), PE-Cyanine7, eBioscience™
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CD244.2 (2B4) Antibody (25-2441-82) in Flow
Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 APC (Product # 17-5941-82) and 0.25 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.25 µg of Anti-Mouse CD244-2 (2B4) PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.
产品信息
25-2441-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
C. elegans,
Mouse
宿主/亚型
Rat
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
eBio244F4
偶联物
激发/发射光谱
569/780 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non-MHC dependent cytotoxicity. Expression is found on natural killer (NK) cells and many NKT cells and CD8+ cells. In C57BL/6 mice, two splice variants exist that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner through signaling through SHP-2, while the short form functions in an activating manner. The ligand for CD244 is CD48 which is expressed on all hematopoietic cells. Recent data suggests that CD244 interactions with CD48, but not CD2 interaction with CD48, is essential for IL-2-driven expansion and activation of murine NK cells.
Reports show CD244 expression can discriminate hematopoietic progenitors. Hematopoietic stem cells (HSC) are highly purified as CD150+CD244-CD48- cells while non-self-renewing multipotent hematopoietic progenitors (MPP) are CD244+CD150-CD48- and the most restricted progenitors are CD48+CD244+CD150-.
eBio244F4 was generated against cell lines expressing CD244. It reacts with the C57BL/6 but not Balb/c due to polymorphisms present in the immunoglobulin V domain of the protein. The epitope recognized by eBio244F4 is different from that of 2B4; therefore these antibodies do not cross-block the binding to the respective epitopes.
Applications Reported: This eBio244F4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio244F4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD244; CD244 long isoform; CD244 short isoform; natural killer cell receptor; Natural killer cell receptor 2B4; NK cell receptor 2B4; NK cell type I receptor protein 2B4; NKR2B4; non MHC restricted killing associated; Non-MHC restricted killing associated; Signaling lymphocytic activation molecule 4; SLAM family member 4; SLAMF4
基因别名: 2B4; C9.1; Cd244; F730046O15Rik; Ly90; NAIL; NKR2B4; Nmrk; SLAMF4
UniProt ID: (Mouse) Q07763
Entrez Gene ID: (Mouse) 18106
