Invitrogen

CD254 (RANK Ligand) Recombinant Rabbit Monoclonal Antibody (CD254-Clone 99)

Name: CD254 (RANK Ligand) Recombinant Rabbit Monoclonal Antibody (CD254-Clone 99)
Brand: Invitrogen
SKU: 704975

高级验证

查看其他52个RANKL (RANK Ligand)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)
高级验证 (1)

CD254 (RANK Ligand) Antibody in Western Blot (WB)

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CD254 (RANK Ligand) Antibody (704975) in WB

Western blot was performed using CD254 (RANK Ligand) Recombinant Rabbit Monoclonal Antibody (CD254-Clone 99) (Product # 704975, 1:400) and a ~30 kDa band corresponding to CD254 (RANK Ligand) was observed in all the cell lines tested that were treated with PTI (1X for 4 hrs). Scraped whole cell extracts (40 µg lysate) of PC-3 (Lane 1), Saos-2 (Lane 2), U-2 OS (Lane 3), and MCF7 (Lane 4) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Produc... View More

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产品信息

704975

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

CD254-Clone 99

抗原

Protein corresponding to human CD254 (RANK Ligand) [aa143-aa317]

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

产品详细信息

Recombinant Rabbit monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD254; H-RANK-L; M-Rank-L; ODF; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; rank-l; RANKL; receptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; srankl; TNF-related activation-induced cytokine; TRANCE; tumor necrosis factor (ligand) superfamily member 11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6B; Tumor necrosis factor ligand superfamily member 11

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基因别名: CD254; hRANKL2; ODF; OPGL; OPTB2; RANKL; sOdf; TNFSF11; TNLG6B; TRANCE

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UniProt ID: (Human) O14788

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Entrez Gene ID: (Human) 8600

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功能

cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding

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