Invitrogen

CD266 (TWEAK Receptor) Monoclonal Antibody (ITEM-4), Biotin, eBioscience™

Name: CD266 (TWEAK Receptor) Monoclonal Antibody (ITEM-4), Biotin, eBioscience\u2122
Brand: Invitrogen
SKU: 13-9018-82

2 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

CD266 (TWEAK Receptor) Antibody in Flow Cytometry (Flow)

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CD266 (TWEAK Receptor) Antibody (13-9018-82) in Flow

Staining of non-transfected (left) and human CD266 transfected (right) L5178Y cells with 0.25 µg of Mouse IgG2b Iso Control (Product # 13-4732-85) (open histogram) or 0.25 µg Anti-Human/Mouse CD266 (TWEAK Receptor) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were analyzed.

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产品信息

13-9018-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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流式细胞分析 (Flow)

0.5 µg/test

功能检测 (Functional)

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产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

ITEM-4

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_466960

产品详细信息

Description: The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line.

The ITEM-4 antibody has been show to have blocking function.

Applications Reported: The ITEM-4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The ITEM-4 antibody has been tested by flow cytometric analysis on human and mouse Fn14 transfected cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TWEAK is a member of the TNF family and was first described as a weak inducer of apoptosis in demonstrate that this relatively small molecule is a fully transformed cell lines. Recently, a receptor for TWEAK was isolated by expression cloning from a HUVEC cell cDNA library. TweakR is a small protein belonging to TNF receptor super family. TweakR is capable of intiating a proliferative signal by binding to TRAF1, 2, and 3. TweakR mRNA is upregulated during tissue regeneration in the aorta and liver. It may also play a role in vascular cell migration and angiogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: CD266; FGF-inducible 14; fibroblast growth factor regulated protein 2; Fibroblast growth factor-inducible immediate-early response protein 14; Fibroblast growth factor-regulated protein 2; Tumor necrosis factor receptor superfamily member 12A; tumor necrosis factor receptor superfamily, member 12A; Tweak-receptor; TweakR; type I transmembrane protein Fn14

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基因别名: AI255180; C87282; CD266; Fgfrp2; FN14; HPIP; TNFRSF12A; TWEAK-R; TWEAKR

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UniProt ID: (Human) Q9NP84, (Mouse) Q9CR75

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Entrez Gene ID: (Human) 51330, (Mouse) 27279

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功能

protein binding transmembrane signal receptor

参与通路