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Invitrogen
CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), Alexa Fluor™ 700, eBioscience™
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CD274 (PD-L1, B7-H1) Antibody (56-5983-42) in Flow
Normal human peripheral blood cells were unstimulated (left) or stimulated with PHA-L overnight (right). Cells were then stained with Mouse IgG1 kappa Isotype Control, Alexa Fluor 700 (Product # 56-4714-81) (blue histogram) or CD274 Monoclonal Antibody, Alexa Fluor 700 (purple histogram). Cells in the lymphocyte gate were used for analysis.
产品信息
56-5983-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
C. elegans
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
MIH1
偶联物
激发/发射光谱
696/719 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The MIH1 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH1 is reported to be a blocking antibody.
Applications Reported: This MIH1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MIH1 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser
靶标信息
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: B7 homolog 1; B7-H1; CD274; CD274 antigen; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1
基因别名: B7-H; B7H1; CD274; PD-L1; PDCD1L1; PDCD1LG1; PDL1
UniProt ID: (Human) Q9NZQ7
Entrez Gene ID: (Human) 29126
immune response
signal transduction
cell surface receptor signaling pathway
positive regulation of cell migration
T cell costimulation
negative regulation of interferon-gamma production
negative regulation of interleukin-10 production
response to cytokine
positive regulation of T cell proliferation
regulation of activated T cell proliferation
negative regulation of activated T cell proliferation
regulation of T cell apoptotic process
toxin transport
negative regulation of tumor necrosis factor superfamily cytokine production
positive regulation of interleukin-10 secretion
