Invitrogen

CD28 Monoclonal Antibody (37.51), Functional Grade, eBioscience™

Name: CD28 Monoclonal Antibody (37.51), Functional Grade, eBioscience\u2122
Brand: Invitrogen
SKU: 16-0281-82

270 篇参考文献

查看其他138个CD28抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

图: 1 / 1

CD28 Antibody (16-0281-82) in Flow

Staining of BALB/c thymocytes with 0.25 µg of Golden Syrian Hamster IgG Isotype Control Functional Grade Purified (Product # 16-4914-85) (open histogram) or 0.25 µg of Anti-Mouse CD28 Functional Grade Purified (filled histogram) followed by Anti-Golden Syrian Hamster IgG FITC (Product # 11-4211-85). Cells in the lymphocyte gate were used for analysis.

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产品信息

16-0281-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫组化 (IHC)

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流式细胞分析 (Flow)

0.5 µg/test

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酶联免疫吸附实验 (ELISA)

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功能检测 (Functional)

Assay-Dependent

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抑制试剂 (IA)

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T细胞活化 (TCA)

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亲和纯化 (AP)

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其他PubMed (Misc)

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产品规格

种属反应

Mouse

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Syrian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

37.51

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_468921

产品详细信息

Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported: The 37.51 antibody has been reported for use in flow cytometric analysis. 37.51 has also been reported in costimulation of T cells in vitro and in vivo .

Applications Tested: The 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。