Invitrogen

CD2AP Monoclonal Antibody (5F8)

Name: CD2AP Monoclonal Antibody (5F8)
Brand: Invitrogen
SKU: MA5-33009

高级验证

1 篇参考文献

查看其他11个CD2AP抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (20)
高级验证 (1)

CD2AP Antibody in Immunocytochemistry (ICC/IF)

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CD2AP Antibody (MA5-33009) in ICC/IF

Immunofluorescence analysis of CD2AP was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD2AP Monoclonal Antibody (5F8) (Product # MA5-33009) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a... View More

CD2AP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD2AP Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. A-431 cells were transfected with CD2-associated protein siRNA and a decrease in signal intensity was observed in Western Blot application using Anti-CD2AP Monoclonal Antibody (5F8) (Product # MA5-33009). {KD}

Immunogen image

Immunogen Image CD2AP Monoclonal Antibody (5F8)

产品信息

MA5-33009

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

亲和纯化 (AP)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

C. elegans

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

5F8

抗原

E.coli-derived human CD2AP recombinant protein (Position: K253-K337).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2802643

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human K562 whole cell, human A431 whole cell, human HEK293 whole cell, human U20S whole cell, human HL-60 whole cell, human MCF-7 whole cell, human Hela whole cell, human PANC-1 whole cell, rat liver tissue, rat testicular tissue, mouse pancreas tissue, mouse testicular tissue, mouse RAW2467 whole cell. IHC: human mammary cancer, human mammary cancer, human colon cancer, human placenta tissue, mouse kidney tissue, rat brain tissue. ICC/IF: A431 cell. Flow: K562 cell, U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Adapter protein CMS; Cas ligand with multiple SH3 domains; Cas ligand with multiple Src homology 3 (SH3) domains; CD2-associated protein; mesenchyme to epithelium transition protein, SH3 domains; Mesenchyme-to-epithelium transition protein with SH3 domains 1; METS-1; SH3 domain-containing adapter protein; SH3 domain-containing adaptor protein METS-1

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基因别名: AL024079; C78928; CD2AP; CMS; METS-1; Mets1

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UniProt ID: (Human) Q9Y5K6, (Mouse) Q9JLQ0

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Entrez Gene ID: (Human) 23607, (Mouse) 12488, (Rat) 316258

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功能

vascular endothelial growth factor receptor binding structural constituent of cytoskeleton protein binding beta-catenin binding protein C-terminus binding SH3 domain binding protein complex binding cadherin binding involved in cell-cell adhesion cadherin binding

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