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Invitrogen
CD305 (LAIR1) Monoclonal Antibody (113), eBioscience™
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-Flow-20170216130641.jpg?time=20220826 "CD305 (LAIR1) Antibody in Flow Cytometry (Flow)")
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CD305 (LAIR1) Antibody (14-3051-82) in Flow
Staining of C57BL/6 splenocytes with 0.125 µg of Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (purple line) or 0.125 µg of Anti-Mouse CD305 (LAIR-1) Purified (blue line) followed by Anti-Armenian Hamster IgG PE (Product # 12-4112-83). Total viable F4/80+ cells were used for analysis.
-Flow-20170216130641.jpg?time=20220826)
-Flow-20170216130641.jpg?time=20220826?time=20220826 "CD305 (LAIR1) Antibody (14-3051-82) in Flow")
产品信息
14-3051-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
C. elegans,
Mouse
宿主/亚型
Armenian hamster
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
113
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: CD305 or Leukocyte-associated Ig-like receptor-1 (LAIR-1) is a 46 kD cell surface molecule that functions as an inhibitory collagen-receptor on the majority of cells of the immune system. The mouse LAIR-1 molecule is expressed primarily on monocytes/macrophages and also on T cells, NK cells and dendritic cells. Unlike human B cells , mouse B cells do not express LAIR-1. The cytoplasmic tail of mouse LAIR-1 can be phosphorylated leading to the recruitment of SH2-containing tyrosine phosphatase(SHP)-2 and C-terminal Src kinase.
Applications Reported: This 113 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).
Applications Tested: This 113 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD305, also known as LAIR1, is a type I transmembrane glycoprotein with an extracellular Ig-like domain and two intracellular ITIMs. It is expressed on a wide range of immune cells, including T, NK, and B cells, monocytes, dendritic cells, eosinophils, basophils, and mast cells. In humans, CD305 is also present on CD34+ hematopoietic progenitor cells and thymocytes. Several isoforms exist due to alternative splicing, with expression varying between cell types. CD305 functions as an inhibitory receptor, playing a key role in regulating immune responses. It blocks cytokine-mediated signaling, cell proliferation, and apoptosis. In NK, B, and T cells, CD305 modulates cytolytic functions and cytokine production, down-regulating IL2 and IFNG in CD4+ T cells while inducing transforming growth factor beta. In B cells, it reduces IgG and IgE production and decreases IL8, IL10, and TNF secretion. Activation of CD305 through tyrosine phosphorylation recruits phosphatases PTPN6 and PTPN11, although it may also function independently of SH2-containing phosphatases. It inhibits differentiation of peripheral blood precursors into dendritic cells and has been shown to induce apoptosis in myeloid leukemia cell lines. Collagens are identified as ligands for CD305, underscoring its role in immune regulation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD305; LAIR-1; Leukocyte-associated immunoglobulin-like receptor 1
基因别名: 5133400O11Rik; BB115266; D7Bwg0421e; Lair-1; Lair1
UniProt ID: (Mouse) Q8BG84
Entrez Gene ID: (Mouse) 52855
