Invitrogen

CD314 (NKG2D) Monoclonal Antibody (1D11), FITC, eBioscience™

Name: CD314 (NKG2D) Monoclonal Antibody (1D11), FITC, eBioscience\u2122
Brand: Invitrogen
SKU: 11-5878-42

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9 篇参考文献

查看其他44个NKG2D (CD314)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (2)

CD314 (NKG2D) Antibody in Flow Cytometry (Flow)

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CD314 (NKG2D) Antibody (11-5878-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD8a eFluor® 450 (Product # 48-0086-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD314 (NKG2D) FITC (right). Cells in the lymphocyte gate were used for analysis.

CD314 (NKG2D) Antibody in Flow Cytometry, Neutralization (Flow, Neu)

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产品信息

11-5878-42

应用

建议稀释比

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流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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中和实验 (Neu)

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产品规格

种属反应

Human

已发表种属

C. elegans, Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

1D11

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2572509

产品详细信息

Description: The 1D11 monoclonal antibody reacts with the human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, gamma delta T cells, CD8+ T cells, and some CD4+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gamma delta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells.

Applications Reported: The 1D11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1D11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

生物信息学

蛋白别名: CD314; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; natural killer cell receptor; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor

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基因别名: CD314; D12S2489E; KLR; KLRK1; NKG2-D; NKG2D

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UniProt ID: (Human) P26718

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Entrez Gene ID: (Human) 22914

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功能

receptor activity protein binding kinase binding carbohydrate binding MHC class Ib receptor activity MHC class I protein binding protein homodimerization activity

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