Search Thermo Fisher Scientific
Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
This monoclonal antibody CSTEM27 reacts with human CD321 (F11R, JAM-1, JAM-A).
Applications Reported: This CSTEM27 antibody has been reported for use in immunocytochemical staining, flow cytometric analysis, and western blot. (Fluorochrome-conjugated CSTEM27 is recommended for use in flow cytometery.)
Applications Tested: This CSTEM27 antibody has been tested by immunocytochemistry of adherent cells. This can be used at less than or equal to 5 µg/mL. This CSTEM27 antibody has also been tested by flow cytometric analysis of human iPSCs. This may be used at 0.25 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
CD321 (F11R, JAM-1, JAM-A) is a cell-surface adhesion molecule belonging to the Ig superfamily of receptors. CD321 is a 32-41 kD glycoprotein, possessing two extracellular Ig-like domains, a transmembrane region, and a short cytosolic tail. The protein assembles at the membrane as a homodimer, and can associate laterally with other membrane proteins including integrins. CD321 is expressed on a variety of cell types, including leukocytes, platelets, erythrocytes, stem cells, epithelial and endothelial cells, and plays a role in mediating cell-cell contact. CD321 can be found in close proximity to several components of epithelial tight junctions, including ZO-1 and occludin. CD321 is involved in the maintenance of epithelial and endothelial barriers, hematopoiesis, angiogenesis, and tumor proliferation, invasion, and metastasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: CD321; F11 R; JAM-1; JAM-A; Junctional adhesion molecule 1; Junctional adhesion molecule A; PAM-1; Platelet adhesion molecule 1; Platelet F11 receptor
基因别名: CD321; F11R; JAM; JAM1; JAMA; JCAM; KAT; PAM-1; UNQ264/PRO301
UniProt ID: (Human) Q9Y624
Entrez Gene ID: (Human) 50848