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Invitrogen
CD337 (NKp30) Monoclonal Antibody (AF29-4D12), PE-eFluor™ 610, eBioscience™
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FIGURE: 1 / 1
CD337 (NKp30) Antibody (61-3379-42) in Flow
Normal human peripheral blood cells were stained with CD56 (NCAM) Monoclonal Antibody, FITC (Product # 11-0566-42) and Mouse IgG1 kappa Isotype Control, PE-eFluor 610 (Product # 61-4714-81) (left) or CD337 (NKp30) Monoclonal Antibody, PE-eFluor 610 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18).
Product Details
61-3379-42
Product Specifications
Species Reactivity
Human,
Non-human primate,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
AF29-4D12
Conjugate
Excitation/Emission Max
565/606 nm
View spectra
Form
liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This AF29-4D12 monoclonal antibody reacts with human CD337, which is also known as NKp30. Expressed on NK cells, CD337 is the predominant member of the natural cytotoxicity receptor family that also includes NKp46 and NKp44. CD337 is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor, virus-infected, and immature dendritic cells. Several ligands for this receptor have been reported, including B7-H6.
The AF29-4D12 antibody has been reported to crossreact to rhesus macaque.
Applications Reported: This AF29-4D12 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This AF29-4D12 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.6 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PE-eFluor™ 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red™). Please make sure that your instrument is capable of detecting this fluorochome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells. The encoded protein interacts with CD3-zeta (CD247), a T-cell receptor. A single nucleotide polymorphism in the 5' untranslated region of this gene has been associated with mild malaria suceptibility. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activating natural killer receptor p30; activating NK-A1 receptor; CD337; lymphocyte antigen 117; Natural cytotoxicity triggering receptor 3; Natural killer cell p30-related protein; natural killer cell receptor; NK-p30
Gene Aliases: 1C7; CD337; LY117; MALS; NCR3; NKp30
UniProt ID: (Human) O14931, (Rhesus monkey) Q8MJ02
Entrez Gene ID: (Human) 259197, (Rhesus monkey) 715574
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
inflammatory response
immune response
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
cell recognition
susceptibility to natural killer cell mediated cytotoxicity
positive regulation of natural killer cell mediated cytotoxicity
regulation of immune response
susceptibility to T cell mediated cytotoxicity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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