Invitrogen

CD34 Polyclonal Antibody

Name: CD34 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-47849

3个已发表图片

3 篇参考文献

查看其他131个CD34抗体

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (2)
已发表图片 (3)

图: 1 / 5

CD34 Antibody (PA5-47849) in WB

Western blot analysis of CD34 in mouse brain (cortex) tissue and rat testis tissue. Samples were incubated in CD34 polyclonal antibody (Product # PA5-47849) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for CD34 at approximately 85‚90 kDa (as indicated). This experiment was conducted under reducing conditions.

CD34 Antibody in Immunohistochemistry (IHC)

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Immunogen image

Immunogen Image CD34 Polyclonal Antibody

产品信息

PA5-47849

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

2.5 µg per million cells

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产品规格

种属反应

Mouse, Rat

已发表种属

Rat

宿主/亚型

Sheep / IgG

分类

Polyclonal

类型

Antibody

抗原

Mouse myeloma cell line NS0-derived recombinant mouse CD34 Thr35-Thr287

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2607331

产品详细信息

In direct ELISAs, less than 3% cross-reactivity with recombinant porcine CD34 is observed.

Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.

靶标信息

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。