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Invitrogen
CD357 (AITR/GITR) Chimeric Recombinant Rabbit Monoclonal Antibody (DTA-1)
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CD357 (AITR/GITR) Chimeric Antibody (MA5-48161) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of GITR in mouse splenocytes using CD357 (AITR/GITR) Chimeric Monoclonal Antibody (Product # MA5-48161). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with CD357 (AITR/GITR) Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (b... View More
产品信息
MA5-48161
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG, lambda
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
DTA-1
抗原
This antibody was raised by immunising Wistar rats with the CD25+CD4+ T cell line.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.02% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Specificity: This antibody is specific for murine GITR, a member of the tumor necrosis factor receptor (TNFR) family that is expressed at low levels on unstimulated T cells, B cells, and macrophages. Upon activation, CD4+ and CD8+ T cells up-regulate GITR expression, whereas immunoregulatory T cells constitutively express high levels of GITR.
Preparation: This antibody was raised by immunising Wistar rats with the CD25+CD4+ T cell line. Hybridoma cells were generated by fusing spleen cells from the immunized rat with P3X63Ag8.653 myeloma cells, assessed for secreting antibodies capable of neutralizing in vitro CD25+CD4+ T cell-mediated suppression of anti-CD3-stimulated T cell proliferation9, and then cloned. This DTA-1 clone secreted a monoclonal rat immunoglobulin G2a (IgG2a) that showed high neutralizing activity.
靶标信息
Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD357; Glucocorticoid-induced TNFR-related protein; PRO364; Tumor necrosis factor receptor superfamily member 18; UNQ319
基因别名: AITR; Gitr; Tnfrsf18
UniProt ID: (Mouse) O35714
Entrez Gene ID: (Mouse) 21936
