Invitrogen

CD36 Monoclonal Antibody (HM36), Super Bright™ 780, eBioscience™

Name: CD36 Monoclonal Antibody (HM36), Super Bright\u2122 780, eBioscience\u2122
Brand: Invitrogen
SKU: 78-0362-82

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说明书

实验方案

问题与答案

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说明书

实验方案

问题与答案

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CD36 Antibody (78-0362-82) in Flow

BALB/c mouse thioglycolate-elicited peritoneal exudate cells were stained with F4/80 Monoclonal Antibody, FITC (Product # 11-4801-82) and 0.25 µg of CD36 Monoclonal Antibody, Super Bright 780.

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CD36 Antibody — Staining of mouse resident peritoneal exudate cells. As expected based on known expression patterns, CD36 is expressed on Small Peritoneal Macrophages (SPM) and Large Peritoneal Macrophages (LPM) but not eosinophils or lymphocytes. Details: C57BL/6 resident peritoneal exudate cells were stained with CD11b (clone M1/70), F4/80 (clone BM8), and either CD36 (clone HM36, colored histograms), or Armenian Hamster IgG Isotype Control (gray histograms). Cells in the indicated color gates were used for analysis. {RE}

Immunogen image

Immunogen Image CD36 Monoclonal Antibody (HM36), Super Bright™ 780, eBioscience™

产品信息

78-0362-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

HM36

抗原

CHO cells transfected with full-length mouse CD36

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偶联物

Super Bright™ 780 Super Bright™ 780 Super Bright™ 780

激发/发射光谱

413/780 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2734973

产品详细信息

Description: This HM36 antibody recognizes mouse CD36, also known as scavenger receptor BIII.

Applications Reported: This HM36 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HM36 antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product # 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product # A10497).

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

靶标信息

CD36, also known as scavenger receptor class B member 3, is a protein that is expressed on the surface of various cell types, including macrophages, platelets, and adipocytes. It plays a role in lipid metabolism, inflammation, and atherosclerosis, and is involved in the recognition and uptake of various ligands such as oxidized low-density lipoproteins, long-chain fatty acids, and apoptotic cells. CD36 is also implicated in the pathogenesis of malaria. The protein encoded by this gene serves as a receptor for thrombospondin in platelets and various cell lines, and is the fourth major glycoprotein of the platelet surface. It binds to collagen, thrombospondin, anionic phospholipids, and oxidized LDL, and directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. Diseases associated with CD36 include Platelet Glycoprotein IV Deficiency and Coronary Heart Disease 7.

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